Monoclonal mouse anti-human brain natriuretic peptide (BNP) Cat.#

4BNP2

Data Sheet

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MAbs
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Alternative in vitro!
Please note that MAb 24C5 is also available as in vitro produced product under Cat.# 4BNP2cc. For immunoassays utilizing this MAb, we recommend selecting the in vitro produced product.

Properties
Cat.#

4BNP2
Unit

mg
MAbs:

24C5, 26E2, 50E1, 50B7, 57H3

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human synthetic BNP (whole molecule) or synthetic BNP peptide 11FGRKMDRISSSS22 (for MAbs 24C5 and 26E2) conjugated with carrier protein.
Specificity:

Human BNP and proBNP.
MAb isotypes:

IgG1 for MAbs 24C5, 50E1, 26E2.
IgG2a for MAbsﾠ 57H3, 50B7
Applications:

BNP and proBNP immunoassay. All MAbs recognize BNP and proBNP in sandwich immunoassay.

MAbs 24C5, 26E2, 50E1, 50B7 and 57H3 react with proBNP and BNP in Western blotting.

Recommended pairs for BNP sandwich immunoassay (capture-detection): 50E1 – 24C5, 50E1 – 26E2, 24C5 – 50B7, 24C5 – 57H3.

Sensitivity of the pair 50E1 – 24C5 is better than 1 pg/ml (synthetic BNP, Bachem). All pairs recognize with high sensitivity the antigen in plasma samples of patients with heart failure.

MAb 50E1 can be used for proBNP detection in pairs with anti-NTproBNP MAbs 16F3 or 18H5 (Cat.# 4NT1).
Purification:

Chromatography on protein A Sepharose
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:

Human ProBNP and proBNP-derived peptides (BNP and NT-proBNP) TechNotes.
Antibodies for New Type of BNP immunoassay.
Antibodies Produced in vitro.
Single Epitope Sandwich assay for BNP – SES-BNP.
References:

See all references here and here.
Search BNP references from PubMed.
Download datasheet as a PDF file below
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