Monoclonal mouse anti-human brain natriuretic peptide (BNP) Cat.# 4BNP2

Data Sheet

This data sheet is provided for information only. Available MAb’s are shown below. Add product to cart by clicking “add to cart”. You can change the amounts on the next page.

Variants
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Properties
Cat.# 4BNP2
MAbs: 24C5, 26E2, 2G9, 50E1, 50B7, 57H3 , 43B12 Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human synthetic BNP (whole molecule) or synthetic BNP peptide 11FGRKMDRISSSS22 (for MAbs 24C5 and 26E2) conjugated with carrier protein.
Unit mg
Applications: BNP and proBNP immunoassay. All MAbs recognize BNP and proBNP in sandwich immunoassay. MAbs 2G9, 24C5, 26E2, 50E1, 50B7, 57H3 and 43B12 react with proBNP and BNP in Western blotting.
Recommended pairs for BNP sandwich immunoassay (capture - detection):  BNP and proBNP immunoassay. All MAbs recognize BNP and proBNP in sandwich immunoassay. MAbs 2G9, 24C5, 26E2, 50E1, 50B7, 57H3 and 43B12 react with proBNP and BNP in Western blotting. Recommended pairs for BNP sandwich immunoassay (capture - detection):  50E1 - 24C5, 50E1 - 26E2, 24C5 - 50B7, 24C5 - 57H3 Sensitivity of the pair 50E1 - 24C5 is better than 1 pg/ml (synthetic BNP, Bachem). All pairs recognize with high sensitivity the antigen in plasma samples of patients with heart failure. MAb 50E1 can be used for proBNP detection in pairs with anti-NTproBNP MAbs 16F3 or 18H5 (Cat.# 4NT1).
Specificity: Human BNP and proBNP.
MAb isotypes: IgG1 for MAbs 2G9, 24C5, 50E1, 26E2 IgG2a for MAbsᅠ43B12, 57H3, 50B7
Purification: Chromatography on protein A Sepharose
Presentation: PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage: + 4 ᄚC
Material safety note: This BNP antibody product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information: SES-BNP information page Human ProBNP and proBNP-derived peptides (BNP and NT-proBNP) TechNotes Antibodies for New Type of BNP immunoassay ヨ モSingle Epitope Sandwichヤ assay TechNotes
References: Search BNP references from PubMed See all BNP References See Other cardiac markers and blood coagulation products