BNP human, antibody

Cat.# 4BNP2 / 4BNP2cc

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Cardiac markers

 

  • Cat.#

    4BNP2 / 4BNP2cc

  • MAbs in vitro (Cat.# 4BNP2cc):

    24C5cc, 50E1cc, 50B7cc, 57H3cc, 100cc, 130cc, 429cc

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of
    Balb/c mice immunized with:
    Synthetic human BNP, whole molecule, conjugated with carrier protein (MAbs 50E1, 50E1cc, 50B7,
    50B7cc, 57H3, 57H3cc, 429cc)
    Synthetic human BNP peptide a.a.r. 3-15 conjugated with carrier protein (MAb 100cc)
    Synthetic human BNP peptide a.a.r. 11-22 conjugated with carrier protein (MAbs 24C5, 24C5cc,
    26E2)
    Recombinant fusion protein containing BNP fragment a.a.r. 5-28 (MAb 130cc)

  • MAbs in vivo (Cat.# 4BNP2):

    24C5, 26E2, 50E1, 50B7, 57H3

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of
    Balb/c mice immunized with:
    Synthetic human BNP, whole molecule, conjugated with carrier protein (MAbs 50E1, 50E1cc, 50B7,
    50B7cc, 57H3, 57H3cc, 429cc)
    Synthetic human BNP peptide a.a.r. 3-15 conjugated with carrier protein (MAb 100cc)
    Synthetic human BNP peptide a.a.r. 11-22 conjugated with carrier protein (MAbs 24C5, 24C5cc,
    26E2)
    Recombinant fusion protein containing BNP fragment a.a.r. 5-28 (MAb 130cc)

  • Specificity:

    Human BNP and proBNP

  • MAb isotypes:

    IgG1 for MAbs 24C5, 24C5cc, 50E1, 50E1cc, 26E2, 130cc, 429cc
    IgG2a for MAbs 57H3, 57H3cc, 50B7, 50B7cc, 100cc

  • Applications:

    BNP and proBNP immunoassay. All MAbs recognize BNP and proBNP in sandwich immunoassay.

    MAbs 24C5, 24C5cc, 26E2, 50E1, 50E1cc, 50B7, 50B7cc, 57H3 and 57H3cc react with proBNP and BNP in Western blotting.

    Recommended pairs for BNP sandwich immunoassay (capture-detection):
    50E1cc – 24C5cc     57H3cc – 429cc
    50E1cc – 26E2        50E1cc – 130cc
    24C5cc – 50B7cc    50E1cc – 100cc
    24C5cc – 57H3cc    100cc – 57H3cc

    Sensitivity of the pair 50E1cc – 24C5cc is better than 1 pg/ml (synthetic BNP, Bachem). All pairs recognize with high sensitivity the antigen in plasma samples of patients with heart failure.

    MAb 50E1cc can be used for proBNP detection in pairs with anti-NTproBNP MAbs 16F3 or 18H5 (Cat.# 4NT1).

  • Purification:

    Chromatography on protein A Sepharose

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

  • References:

    See all references here and here.
    Search BNP references from PubMed.

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BNP human, antibody (MAb: )

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