Monoclonal mouse anti-human brain natriuretic peptide (BNP) Cat.#

4BNP2 / 4BNP2cc

Data Sheet

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Properties
Unit

mg
Cat.#

4BNP2 / 4BNP2cc
MAbs in vivo (Cat.# 4BNP2):

24C5, 26E2, 50E1, 50B7, 57H3

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with:
Synthetic human BNP, whole molecule, conjugated with carrier protein (MAbs 50E1, 50B7, 57H3)
Synthetic human BNP peptide a.a.r. 11-22 conjugated with carrier protein (MAbs 24C5, 26E2)
MAbs in vitro (Cat.# 4BNP2cc):

24C5cc, 50E1cc, 50B7cc, 100cc, 130cc, 429cc

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with:
Synthetic human BNP, whole molecule, conjugated with carrier protein (MAbs 50E1cc, 50B7cc, 429cc)
Synthetic human BNP peptide a.a.r. 3-15 conjugated with carrier protein (MAb 100cc)
Synthetic human BNP peptide a.a.r. 11-22 conjugated with carrier protein (MAbs 24C5cc)
Recombinant fusion protein containing BNP fragment a.a.r. 5-28 (MAb 130cc)
Specificity:

Human BNP and proBNP.
MAb isotypes:

IgG1 for MAbs 24C5, 24C5cc, 50E1, 50E1cc, 26E2, 130cc, 429cc
IgG2a for MAbsﾠ 57H3, 50B7, 50B7cc, 100cc
Applications:

BNP and proBNP immunoassay. All MAbs recognize BNP and proBNP in sandwich immunoassay.

MAbs 24C5, 24C5cc, 26E2, 50E1, 50E1cc, 50B7, 50B7cc and 57H3 react with proBNP and BNP in Western blotting.

Recommended pairs for BNP sandwich immunoassay (capture-detection):
50E1cc – 24C5cc 57H3 – 429cc
50E1cc – 26E2 50E1cc – 130cc
24C5cc – 50B7cc 50E1cc – 100cc
24C5cc – 57H3 100cc – 57H3
Sensitivity of the pair 50E1cc – 24C5cc is better than 1 pg/ml (synthetic BNP, Bachem). All pairs recognize with high sensitivity the antigen in plasma samples of patients with heart failure.
MAb 50E1cc can be used for proBNP detection in pairs with anti-NTproBNP MAbs 16F3 or 18H5
(Cat.# 4NT1).
Purification:

Chromatography on protein A Sepharose
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:

Human ProBNP and proBNP-derived peptides (BNP and NT-proBNP) TechNotes
Antibodies for New Type of BNP immunoassay
ProBNP, BNP and NT-proBNP Antigens
Antibodies Produced in vitro
Single Epitope Sandwich assay for BNP – SES-BNP
References:

See all references here and here.
Search BNP references from PubMed.
Download datasheet as a PDF file below
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