Recombinant human proBNP, glycosylated Cat.#
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Expressed in a mammalian cell line
Synthetic DNA fragment encoding His27-His134 part of full-length human proBNP (UniProtKB accession number P16860) was used for recombinant protein expression.
Protein is partially glycosylated. On the SDS-PAGE the protein has apparent molecular weight between 20 and 25 kDa.
Calibrator or standard in BNP or proBNP immunoassays
Purity > 95% (Tricine-SDS-PAGE in reducing conditions).
ProBNP concentration is determined spectrophotometrically
Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of proBNP molecule.
Immunoaffinity purification followed by ion-exchange chromatography
Lyophilized from PBS, pH 7.4
It is recommended to reconstitute this product with deionized water to its initial concentration.
Lyophilized -20°C (-15…-30 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
|Material safety note:||
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
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