Expressed in a mammalian cell line
Synthetic DNA fragment encoding His27-His134 part of full-length human proBNP (UniProtKB accession number P16860) was used for recombinant protein expression.
Protein is partially glycosylated. On the SDS-PAGE the protein has apparent molecular weight between 20 and 25 kDa.
Calibrator or standard in BNP or proBNP immunoassays
Purity > 95% (Tricine-SDS-PAGE in reducing conditions).
ProBNP concentration is determined spectrophotometrically
Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of proBNP molecule.
Immunoaffinity purification followed by ion-exchange chromatography
Lyophilized from PBS, pH 7.4
It is recommended to reconstitute this product with deionized water to its initial concentration.
Lyophilized -20°C (-15…-30 °C allowed)
Reconstituted -70 °C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the productafter reconstitution.
To prepare diluted samples of proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01% Tween 40, 0.5% bovine serum albumin).
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.