Recombinant human proBNP, glycosylated Cat.#

8GBP3

Data Sheet

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Properties
Cat.#

8GBP3

Unit

mg

Source:

Expressed in a mammalian cell line

Description:

Synthetic DNA fragment encoding His27-His134 part of full-length human proBNP (UniProtKB accession number P16860) was used for recombinant protein expression.

Protein is partially glycosylated. On the SDS-PAGE the protein has apparent molecular weight between 20 and 25 kDa.

Applications:

Calibrator or standard in BNP or proBNP immunoassays

Analysis:

Purity > 95% (Tricine-SDS-PAGE in reducing conditions).

ProBNP concentration is determined spectrophotometrically

Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of proBNP molecule.

Purification:

Immunoaffinity purification followed by ion-exchange chromatography

Presentation:

Lyophilized from PBS, pH 7.4

It is recommended to reconstitute this product with deionized water to its initial concentration.

Storage:

Lyophilized -20°C (-15…-30 °C allowed)
Reconstituted -70 °C (-65 … -80 °C allowed)

Other information:

Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

References:

Search references from PubMed

Download datasheet as a PDF file below