15F11cc, 13G12cc, 15C4cc, 24E11cc, 29D12cc, 18H5cc, NT34cc
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 1-12 (MAb 29D12cc), a.a.r. 13-27 (MAbs 15F11cc, 13G12cc, 18H5cc), a.a.r. 22-36 (MAb NT34cc) and a.a.r. 61-76 (MAbs 24E11cc, 15C4cc). The peptides used for immunization were conjugated with carrier protein.
Human NT-proBNP and proBNP.
IgG1 for MAbs 18H5cc, NT34cc
IgG2a for MAbs 29D12cc, 13G12cc, 24E11cc
IgG2b for MAbs 15F11cc, 15C4cc
NT-proBNP and proBNP immunoassay. All MAbs are working in Western Blotting.
Best pairs for sandwich immunoassay (capture-detection): 15F11cc – 24E11cc, 15C4cc – 29D12cc, 15C4cc – 13G12cc, 15C4cc – 18H5cc, 29D12cc-NT34cc. These pairs recognize the antigen in blood of patients with heart failure, unstable angina and myocardial infarction.
MAbs specific to peptides 5-12 (MAb 29D12cc), 13-24 (MAb 15F11cc), 15-20 (MAbs 13G12cc, 18H5cc) and 25-34 (MAb NT34cc) recognize circulating proBNP and can be used for the development of quantitative proBNP assays in pairs with anti-BNP MAbs (Cat.# 4BNP2). The best pair (capture-detection):
50E1 (Cat.# 4BNP2) – 18H5cc (Cat.# 4NT1cc)
Chromatography on protein A Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN3)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.