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Monoclonal mouse anti- human N-terminal proBNP (NT-proBNP), in vitro Cat.#

4NT1cc

Data Sheet

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Properties
Unit

mg

Cat.#

4NT1cc

MAbs:

15F11cc, 13G12cc, 15C4cc, 24E11cc, 29D12cc, 18H5cc

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 1-12 (MAb 29D12cc), a.a.r. 13-27 (MAbs 15F11cc, 13G12cc, 18H5cc) and a.a.r. 61-76 (MAbs 24E11cc, 15C4cc). The peptides used for immunization were conjugated with carrier protein.

Specificity:

Human NT-proBNP and proBNP.

MAb isotypes:

IgG1 for MAb 18H5cc

IgG2a for MAbs 29D12cc, 13G12cc, 24E11cc

IgG2b for MAbs 15F11cc, 15C4cc

Applications:

NT-proBNP and proBNP immunoassay. All MAbs are working in Western Blotting.

Best pairs for sandwich immunoassay (capture-detection): 15F11cc – 24E11cc, 15C4cc – 29D12cc, 15C4cc – 13G12cc, 15C4cc – 18H5cc. These pairs recognize the antigen in blood of patients with heart failure, unstable angina and myocardial infarction.

MAbs specific to peptides 5-12 (MAb 29D12cc) and 13-27 (MAbs 15F11cc, 13G12cc, 18H5cc) recognize circulating proBNP and can be used for the development of quantitative proBNP assays in pairs with anti-BNP MAbs (Cat.# 4BNP2). The best pair (capture-detection):

50E1 (Cat.# 4BNP2) – 18H5cc (Cat.# 4NT1cc)

Purification:

Chromatography on protein A Sepharose

Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN3)

Storage:

+4 °C (+2 … +8 °C allowed)

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Download datasheet as a PDF file below
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