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Cat.#
4NT1 / 4NT1cc
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Recombinant MAbs: (Cat.# 4NT1cc):
NT13, NT45, NT46 Recombinant monoclonal antibody expressed in a mammalian cell line. Full-size IgG sequence derived from rabbit B cells.
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MAbs in vitro (Cat.# 4NT1cc):
5B6cc, 29D12cc, 15F11cc, 13G12cc, 18H5cc, 7B5cc, NT34cc, 11D1cc, 16E6cc, 15C4cc, 24E11cc Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice. Normal 0 false false false en-FI X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:8.0pt; mso-para-margin-left:0cm; line-height:107%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-fareast-language:EN-US;}
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MAbs in vivo (Cat.# 4NT1)
16F3, 15D7, 28F8 Mouse monoclonal antibody produced in ascites. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.
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Immunogens
Synthetic peptides corresponding to different regions of human NT-proBNP conjugated with a carrier protein.
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Specificity:
Human NT-proBNP and proBNP. Epitopes:
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MAb isotypes:
IgG for NT13, NT45, NT46IgG1 for 5B6cc, 18H5cc, 7B5cc, NT34cc, 11D1cc, 16E6cc, 16F3, 15D7IgG2a for 29D12cc, 13G12cc, 24E11cc, 28F8IgG2b for 15F11cc, 15C4cc Normal 0 false false false en-FI X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:8.0pt; mso-para-margin-left:0cm; line-height:107%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-fareast-language:EN-US;}
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Applications:
NT-proBNP and proBNP immunoassay. MAbs are working in Western Blotting. Recommended pairs for sandwich immunoassay: MAbs specific to peptides 5-12, 13-24, 15-20, 25-34, 27-31 and 15-21 recognize circulating proBNP and can be used for the development of quantitative proBNP assays in pairs with anti-BNP MAbs. The best pairs are: (Anti-BNP MAbs 50E1cc is under Cat.# 4BNP2cc)
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Purification:
Protein A chromatography
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Presentation:
PBS, pH 7.4, 0.09 % sodium azide (NaN Normal 0 21 false false false FI X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:8.0pt; mso-para-margin-left:0cm; line-height:107%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-fareast-language:EN-US;} ₃)
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Storage:
+4 °C (+2 … +8 °C allowed)
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Material safety note:
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material. Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
