Special Offer! Evaluate MAbs, 0,5mg of each FOC.
When ordering online, write CARDIAC to the remarks field. Select invoice or prepayment invoice and submit your order. We will make the final FOC order confirmation for 0,5 mg and send it to your inbox.
This offer is only available to end users. Offer valid until 08-12/22
Institutes, universities, and distributors are not included in this promotion. Shipping and handling costs will still apply. This is a one-time evaluation possibility. Feedback about the evaluation results is expected. You can get more details by contacting HyTest at email@example.com
4NT1 / 4NT1cc
5B6cc, 29D12cc, 15F11cc, 13G12cc, 18H5cc, 7B5cc, NT34cc, 11D1cc, 16E6cc, 15C4cc, 24E11cc
16F3, 15D7, 28F8
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 1-12 (5B6cc, 29D12cc), a.a.r. 13-27 (15F11cc, 13G12cc, 18H5cc, 7B5cc, 16F3), a.a.r. 22-36 (NT34cc), a.a.r. 28-45 (11D1cc, 16E6cc), a.a.r. 46-60 (15D7) and a.a.r. 61-76 (24E11cc, 28F8, 15C4cc). The peptides used for immunization were conjugated with carrier protein.
Human NT-proBNP and proBNP.
IgG1 for 5B6cc, 18H5cc, 7B5cc, NT34cc, 11D1cc, 16E6cc, 16F3, 15D7
IgG2a for 29D12cc, 13G12cc, 24E11cc, 28F8
IgG2b for 15F11cc, 15C4cc
NT-proBNP and proBNP immunoassay. All MAbs are working in Western Blotting.Best pairs for sandwich immunoassay (capture-detection): 15F11cc – 24E11cc, 15C4cc – 29D12cc, 15C4cc – 13G1cc2, 15C4cc – 18H5cc, 29D12cc – NT34ccThese pairs recognize the antigen in blood of patients with heart failure, unstable angina and myocardial infarction.MAbs specific to peptides 5-1 2 (29D12cc), 13-2 4 (15F11cc), 15-20 (13G12cc, 18H5cc, 16F3), 25-34 (NT34cc) and 15-21 (7B5cc) recognize circulating proBNP and can be used for the development of quantitative proBNP assays in pairs with anti-BNP MAbs (Cat.# 4BNP2 / 4BNP2cc).
The best pairs (capture-detection):
50E1cc – 16F3
50E1cc – 18H5cc
(50E1cc is under Cat.# 4BNP2cc)
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
(Catalogue number and MAb: 4NT1 / 4NT1cc-)