Alternatives in vitro!
Please note that MAbs 15F11, 13G12, 15C4, 24E11, 29D12 and 18H5 are also available as in vitro produced products under Cat.#4NT1cc. For immunoassays utilizing of these MAbs, we recommend selecting the in vitro produced product.
5B6, 15F11, 7B5, 13G12, 11D1, 16E6, 15D7, 15C4, 24E11, 28F8, 29D12, 18H5, 16F3
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 1-12 (MAbs 5B6, 29D12), a.a.r. 13-27 (MAbs 15F11, 13G12, 18H5, 7B5, 16F3), a.a.r. 28-45 (MAbs 11D1, 16E6), a.a.r. 46-60 (MAb 15D7) and a.a.r. 61-76 (MAbs 24E11, 28F8, 15C4). The peptides used for immunization were conjugated with carrier protein.
Human NT-proBNP and proBNP.
IgG1 for MAbs 11D1, 5B6, 15D7, 7B5, 16E6, 18H5, 16F3
IgG2a for MAbs 29D12, 13G12, 24E11, 28F8
IgG2b for MAbs 15F11, 15C4
NT-proBNP and proBNP immunoassay. All MAbs are working in Western Blotting.
Best pairs for sandwich immunoassay (capture - detection): 15F11 - 24E11, 15C4 - 29D12, 15C4 - 13G12, 15C4 - 18H5. These pairs recognize the antigen in blood of patients with heart failure, unstable angina and myocardial infarction.
MAbs specific to peptides 5-12 (MAb 29D12), 13-24 (MAb 15F11), 15-20 (MAbs 13G12, 18H5, 16F3) and 15-21 (MAb 7B5) recognize circulating proBNP and can be used for the development of quantitative proBNP assays in pairs with anti-BNP MAbs (Cat.# 4BNP2). The best pairs (coating - conjugate):
Chromatography on protein A Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.