NT-proBNP human antibody

Cat.# 4NT1 / 4NT1cc

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Cardiac markers
  • Cat.#

    4NT1 / 4NT1cc

  • MAbs in vitro (Cat.# 4NT1cc):

    5B6cc, 29D12cc, 15F11cc, 13G12cc, 18H5cc, 7B5cc, NT34cc, 11D1cc, 16E6cc, 15C4cc, 24E11cc

  • MAbs in vivo (Cat.# 4NT1)

    16F3, 15D7, 28F8

     

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 1-12 (5B6cc, 29D12cc), a.a.r. 13-27 (15F11cc, 13G12cc, 18H5cc, 7B5cc, 16F3), a.a.r. 22-36 (NT34cc), a.a.r. 28-45 (11D1cc, 16E6cc), a.a.r. 46-60 (15D7) and a.a.r. 61-76 (24E11cc, 28F8, 15C4cc). The peptides used for immunization were conjugated with carrier protein.

  • Specificity:

    Human NT-proBNP and proBNP.

  • MAb isotypes:

     

    IgG1 for 5B6cc, 18H5cc, 7B5cc, NT34cc, 11D1cc, 16E6cc, 16F3, 15D7

    IgG2a for 29D12cc, 13G12cc, 24E11cc, 28F8

    IgG2b for 15F11cc, 15C4cc

     

  • Applications:

    NT-proBNP and proBNP immunoassay. All MAbs are working in Western Blotting.Best pairs for sandwich immunoassay (capture-detection): 15F11cc – 24E11cc, 15C4cc – 29D12cc, 15C4cc – 13G1cc2, 15C4cc – 18H5cc, 29D12cc – NT34ccThese pairs recognize the antigen in blood of patients with heart failure, unstable angina and myocardial infarction.MAbs specific to peptides 5-1 2 (29D12cc), 13-2 4 (15F11cc), 15-20 (13G12cc, 18H5cc, 16F3), 25-34 (NT34cc) and 15-21 (7B5cc) recognize circulating proBNP and can be used for the development of quantitative proBNP assays in pairs with anti-BNP MAbs (Cat.# 4BNP2 / 4BNP2cc).

    The best pairs (capture-detection):

    50E1cc – 16F3

    50E1cc – 18H5cc

    (50E1cc is under Cat.# 4BNP2cc)

     

  • Purification:

    Protein A chromatography

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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NT-proBNP human antibody

(Catalogue number and MAb: 4NT1 / 4NT1cc-)

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NT-proBNP human antibody

(Catalogue number and MAb: 4NT1 / 4NT1cc-)

Evaluation samples available. You can save up to 50%*

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*The usual shipping and handling costs will still apply. The sample opportunity is a one-time evaluation possibility and you can get more details by contacting HyTest customer service.
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