Monoclonal mouse anti- human N-terminal proBNP (NT-proBNP) Cat.#

4NT1

Data Sheet

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Alternatives in vitro!
Please note that MAbs 15F11, 13G12, 15C4, 24E11, 29D12 and 18H5 are also available as in vitro produced products under Cat.#4NT1cc. For immunoassays utilizing of these MAbs, we recommend selecting the in vitro produced product.

Properties
Unit

mg
Cat.#

4NT1
MAbs:

5B6, 15F11, 7B5, 13G12, 11D1, 16E6, 15D7, 15C4, 24E11, 28F8, 29D12, 18H5, 16F3

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 1-12 (MAbs 5B6, 29D12), a.a.r. 13-27 (MAbs 15F11, 13G12, 18H5, 7B5, 16F3), a.a.r. 28-45 (MAbs 11D1, 16E6), a.a.r. 46-60 (MAb 15D7) and a.a.r. 61-76 (MAbs 24E11, 28F8, 15C4). The peptides used for immunization were conjugated with carrier protein.
Specificity:

Human NT-proBNP and proBNP.
MAb isotypes:

IgG1 for MAbs 11D1, 5B6, 15D7, 7B5, 16E6, 18H5, 16F3
IgG2a for MAbs 29D12, 13G12, 24E11, 28F8
IgG2b for MAbs 15F11, 15C4
Applications:

NT-proBNP and proBNP immunoassay. All MAbs are working in Western Blotting.

Best pairs for sandwich immunoassay (capture - detection):  15F11 - 24E11, 15C4 - 29D12, 15C4 - 13G12, 15C4 - 18H5.  These pairs recognize the antigen in blood of patients with heart failure, unstable angina and myocardial infarction.

MAbs specific to peptides 5-12 (MAb 29D12), 13-24 (MAb 15F11), 15-20 (MAbs 13G12, 18H5, 16F3) and 15-21 (MAb 7B5) recognize circulating proBNP and can be used for the development of quantitative proBNP assays in pairs with anti-BNP MAbs (Cat.# 4BNP2). The best pairs (coating - conjugate):

50E1 (Cat.# 4BNP2) - 16F3(Cat.# 4NT1)
50E1 (Cat.# 4BNP2) - 18H5 (Cat.# 4NT1)
7B5 (Cat. # 4NT1) - 2G9 (Cat. # 4BNP2).
Purification:

Chromatography on protein A Sepharose
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:

Human ProBNP and proBNP-derived peptides (BNP and NT-proBNP) TechNotes
ProBNP, BNP and NT-proBNP Antigens
References:

See Other cardiac markers and blood coagulation products (amino terminal human brain natriuretic peptide precursor).
See all NT-proBNP ﾠReferences.
Search NT-proBNP references from PubMed.
Download datasheet as a PDF file below
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