NT-proBNP human antibody

Cat.# 4NT1 / 4NT1cc

Special Offer! Evaluate MAbs, 0,5mg of each FOC.

When ordering online, write CARDIAC to the remarks field. Select invoice or prepayment invoice and submit your order. We will make the final FOC order confirmation for 0,5 mg and send it to your inbox.

Terms:
This offer is only available to end users. Offer valid until 08-12/22
Institutes, universities, and distributors are not included in this promotion. Shipping and handling costs will still apply. This is a one-time evaluation possibility. Feedback about the evaluation results is expected. You can get more details by contacting HyTest at hytest@hytest.fi

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Cardiac markers
  • Cat.#

    4NT1 / 4NT1cc

  • MAbs in vitro (Cat.# 4NT1cc):

    5B6cc, 29D12cc, 15F11cc, 13G12cc, 18H5cc, 7B5cc, NT34cc, 11D1cc, 16E6cc, 15C4cc, 24E11cc

  • MAbs in vivo (Cat.# 4NT1)

    16F3, 15D7, 28F8

     

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 1-12 (5B6cc, 29D12cc), a.a.r. 13-27 (15F11cc, 13G12cc, 18H5cc, 7B5cc, 16F3), a.a.r. 22-36 (NT34cc), a.a.r. 28-45 (11D1cc, 16E6cc), a.a.r. 46-60 (15D7) and a.a.r. 61-76 (24E11cc, 28F8, 15C4cc). The peptides used for immunization were conjugated with carrier protein.

  • Specificity:

    Human NT-proBNP and proBNP.

  • MAb isotypes:

     

    IgG1 for 5B6cc, 18H5cc, 7B5cc, NT34cc, 11D1cc, 16E6cc, 16F3, 15D7

    IgG2a for 29D12cc, 13G12cc, 24E11cc, 28F8

    IgG2b for 15F11cc, 15C4cc

     

  • Applications:

    NT-proBNP and proBNP immunoassay. All MAbs are working in Western Blotting.Best pairs for sandwich immunoassay (capture-detection): 15F11cc – 24E11cc, 15C4cc – 29D12cc, 15C4cc – 13G1cc2, 15C4cc – 18H5cc, 29D12cc – NT34ccThese pairs recognize the antigen in blood of patients with heart failure, unstable angina and myocardial infarction.MAbs specific to peptides 5-1 2 (29D12cc), 13-2 4 (15F11cc), 15-20 (13G12cc, 18H5cc, 16F3), 25-34 (NT34cc) and 15-21 (7B5cc) recognize circulating proBNP and can be used for the development of quantitative proBNP assays in pairs with anti-BNP MAbs (Cat.# 4BNP2 / 4BNP2cc).

    The best pairs (capture-detection):

    50E1cc – 16F3

    50E1cc – 18H5cc

    (50E1cc is under Cat.# 4BNP2cc)

     

  • Purification:

    Protein A chromatography

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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NT-proBNP human antibody

(Catalogue number and MAb: 4NT1 / 4NT1cc-)

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(Add your PO number here. Also if agreed on special conditions - e.g. quoted bulk prices - state it here and we will craft the final order confirmation accordingly. If you want some item to be sent in several vials, please write it here.)

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Sample Request

NT-proBNP human antibody

(Catalogue number and MAb: 4NT1 / 4NT1cc-)

Evaluation samples available. You can save up to 50%*

Information

*The usual shipping and handling costs will still apply. The sample opportunity is a one-time evaluation possibility and you can get more details by contacting HyTest customer service.

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