NT-proBNP recombinant human

Cat.# 8NT2

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Cardiac markers
  • Cat.#

    8NT2

  • Source:

    Expressed in E.coli

  • Application:

    Calibrator or standard in immunoassays, immunogen for antibody production.

  • Analysis:

    Purity > 95 % (Tricine-SDS-PAGE).

    Concentration is determined spectrophotometrically.

    Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of NT-proBNP molecule.

    Predicted molecular mass 8589 Da confirmed by MALDI-MS.

    Recombinant human NT-proBNP contains additional methionine residue at the N-terminus (in comparison with native NT-proBNP).

  • Purification:

    Immunoaffinity purification followed by ion-exchange chromatography.

  • Presentation:

    10 mM potassium phosphate, pH 7.4, 150 mM NaCl.

  • Storage:

    -70 °C (-65 … -80 °C allowed)

  • Other information:

    Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.

    To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine serum albumin).

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

  • Additional product information:
  • References:

    See other cardiac markers and blood coagulation products.
    See all NT-proBNP References.
    Search other references from PubMed.

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NT-proBNP recombinant human (MAb: )

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