NT-proBNP recombinant human

Cat.# 8NT2

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  • Cat.#
    8NT2
  • Source:
    Expressed in E.coli
  • Application:
    Calibrator or standard in immunoassays, immunogen for antibody production.
  • Analysis:
    Purity > 95 % (Tricine-SDS-PAGE). Concentration is determined spectrophotometrically. Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of NT-proBNP molecule. Predicted molecular mass 8589 Da confirmed by MALDI-MS. Recombinant human NT-proBNP contains additional methionine residue at the N-terminus (in comparison with native NT-proBNP).
  • Purification:
    Immunoaffinity purification followed by ion-exchange chromatography.
  • Presentation:
    10 mM potassium phosphate, pH 7.4, 150 mM NaCl.
  • Storage:
    -70 °C (-65 … -80 °C allowed)
  • Other information:
    Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing. To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine serum albumin).
  • Material safety note:
    This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
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NT-proBNP recombinant human () —
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