Expressed in E.coli
Calibrator or standard in immunoassays, immunogen for antibody production.
Purity > 95 % (Tricine-SDS-PAGE).
Concentration is determined spectrophotometrically.
Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of NT-proBNP molecule.
Predicted molecular mass 8589 Da confirmed by MALDI-MS.
Recombinant human NT-proBNP contains additional methionine residue at the N-terminus (in comparison with native NT-proBNP).
Immunoaffinity purification followed by ion-exchange chromatography.
10 mM potassium phosphate, pH 7.4, 150 mM NaCl.
-70 °C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.
To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine serum albumin).
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.