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Human recombinant NT-proBNP (amino terminal human brain natriuretic peptide precursor) Cat.#

8NT2

Data Sheet

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Properties
Cat.#

8NT2

Source:

Expressed in E.coli

Application:

Calibrator or standard in immunoassays, immunogen for antibody production.

Analysis:

Purity > 95 % (Tricine-SDS-PAGE).

Concentration is determined spectrophotometrically.

Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of NT-proBNP molecule.

Predicted molecular mass 8589 Da confirmed by MALDI-MS.

Recombinant human NT-proBNP contains additional methionine residue at the N-terminus (in comparison with native NT-proBNP).

Purification:

Immunoaffinity purification followed by ion-exchange chromatography.

Presentation:

10 mM potassium phosphate, pH 7.4, 150 mM NaCl.

Storage:

-70 °C (-65 … -80 °C allowed)

Other information:

Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.

To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine serum albumin).

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Additional product information:

Antibodies for New Type of BNP immunoassay TechNotes.
ProBNP and proBNP-derived peptides (BNP and NT-proBNP) TechNotes.

References:

See other cardiac markers and blood coagulation products.
See all NT-proBNP References.
Search other references from PubMed.

Download datasheet as a PDF file below
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