Serum amyloid A (SAA), animal, antibody

Cat.# 4VS4

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Veterinary
  • Cat.#

    4VS4

  • MAbs in vitro:

    SAA19cc, SAA21cc, VSA31cc, VSA34cc, VSA38cc

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with either human SAA (SAA19cc, SAA21cc), or canine SAA (VSA34cc, VSA31cc, VSA38cc)

  • MAbs in vivo:

    VSA34, SAA19, SAA21, SAA11, VSA31, VSA38, VSA2, VSA43

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with either human SAA (SAA19, SAA21, SAA11), or canine SAA (VSA34, VSA31, VSA38), or synthetic peptides derived from the region 79-104 a.a.r. of canine SAA (VSA2, VSA43).

  • Specificity:

    Canine SAA (all MAbs)
    Feline SAA (VSA34, VSA34cc, SAA19, SAA19cc, VSA31, VSA31cc, VSA38, VSA38cc, SAA21, SAA21cc, SAA11)
    Equine SAA (all MAbs)
    Human SAA (MAbs VSA31, VSA31cc, VSA38, VSA38cc, SAA11)

  • MAb isotypes:

    IgG1 for MAb VSA2
    IgG2a for MAbs SAA19, SAA19cc, VSA31, VSA31cc, VSA38, VSA38cc
    IgG2b for MAbs VSA34, VSA34cc, SAA21, SAA21cc, VSA43, SAA11

  • Applications:

    Recommended pairs for sandwich immunoassay (capture – detection):
    SAA19cc – VSA34cc: canine, feline, equine
    SAA21cc – VSA34cc: canine, feline, equine
    VSA2 – VSA38cc: canine, equine
    VSA2 – VSA31cc: canine, equine
    VSA38cc – VSA43: canine, equine
    All MAbs react with SAA in Western blotting.

  • Purification:

    Protein A chromatography

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN) for MAbs SAA11 SAA19cc, SAA19, VSA2, VSA31cc, VSA31, VSA34cc, VSA34, VSA38, VSA38cc, VSA43
    50 mM citrate, 150 mM NaCl, pH 6.0, 0,09% azide (NaN) for MAbs SAA21cc, SAA21

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

  • Other information:

    We recommend to avoid using bovine serum albumin (BSA) as a buffer component or blocking agent for SAA immunoassay. In buffers, BSA can be replaced with 1% casein.

    Immunoassay plates blocking with casein is needed to prevent non-specific binding of SAA to the wells of an immunoassay plate.

    For pairs SAA19cc – VSA34cc and SAA21cc – VSA34cc, we recommend to start assay optimization using 0.01% CHAPS in antigen dilution and washing buffers. According to our data, Tween 20 lowered the signal in the wells when it was used in concentration 0.05-0.1% in antigen dilution buffer.
    When lower concentration of Tween 20 was used (0.005-0.025%), non-specific binding of SAA to the wells of an immunoassay plate was observed.

    For pairs VSA2 – VSA38cc, VSA2 – VSA31cc and VSA38cc – VSA43, we recommend to use antigen dilution buffer containing 1% casein and 0.05-0.1% Tween 20.

     

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Serum amyloid A (SAA), animal, antibody (MAb: )

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Serum amyloid A (SAA), animal, antibody (MAb: )

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