SAA19cc, SAA21cc, VSA31cc, VSA34cc, VSA38cc
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with either human SAA (SAA19cc, SAA21cc), or canine SAA (VSA34cc, VSA31cc, VSA38cc)
VSA34, SAA19, SAA21, SAA11, VSA31, VSA38, VSA2, VSA43
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with either human SAA (SAA19, SAA21, SAA11), or canine SAA (VSA34, VSA31, VSA38), or synthetic peptides derived from the region 79-104 a.a.r. of canine SAA (VSA2, VSA43).
Canine SAA (all MAbs)
Feline SAA (VSA34, VSA34cc, SAA19, SAA19cc, VSA31, VSA31cc, VSA38, VSA38cc, SAA21, SAA21cc, SAA11)
Equine SAA (all MAbs)
Human SAA (MAbs VSA31, VSA31cc, VSA38, VSA38cc, SAA11)
IgG1 for MAb VSA2
IgG2a for MAbs SAA19, SAA19cc, VSA31, VSA31cc, VSA38, VSA38cc
IgG2b for MAbs VSA34, VSA34cc, SAA21, SAA21cc, VSA43, SAA11
Recommended pairs for sandwich immunoassay (capture – detection):
SAA19cc – VSA34cc: canine, feline, equine
SAA21cc – VSA34cc: canine, feline, equine
VSA2 – VSA38cc: canine, equine
VSA2 – VSA31cc: canine, equine
VSA38cc – VSA43: canine, equine
All MAbs react with SAA in Western blotting.
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃) for MAbs SAA11 SAA19cc, SAA19, VSA2, VSA31cc, VSA31, VSA34cc, VSA34, VSA38, VSA38cc, VSA43
50 mM citrate, 150 mM NaCl, pH 6.0, 0,09% azide (NaN₃) for MAbs SAA21cc, SAA21
+4 °C (+2 … +8 °C allowed)
We recommend to avoid using bovine serum albumin (BSA) as a buffer component or blocking agent for SAA immunoassay. In buffers, BSA can be replaced with 1% casein.
Immunoassay plates blocking with casein is needed to prevent non-specific binding of SAA to the wells of an immunoassay plate.
For pairs SAA19cc – VSA34cc and SAA21cc – VSA34cc, we recommend to start assay optimization using 0.01% CHAPS in antigen dilution and washing buffers. According to our data, Tween 20 lowered the signal in the wells when it was used in concentration 0.05-0.1% in antigen dilution buffer.
When lower concentration of Tween 20 was used (0.005-0.025%), non-specific binding of SAA to the wells of an immunoassay plate was observed.
For pairs VSA2 – VSA38cc, VSA2 – VSA31cc and VSA38cc – VSA43, we recommend to use antigen dilution buffer containing 1% casein and 0.05-0.1% Tween 20.
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
(Catalogue number and MAb: 4VS4-)