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Monoclonal mouse anti-serum amyloid A (SAA) Cat.#

4SA11

Data Sheet

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Properties
Unit

mg
Cat.#

4SA11
MAbs:

SAA1, SAA6, SAA15, VSA6, VSA25

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with either human SAA (SAA1, SAA6, SAA15), or synthetic peptides corresponding to the regions 23-29 a.a.r. (VSA25) and 72-86 a.a.r. (VSA6) of human SAA.
Specificity:

Human SAA. All MAbs cross-react with canine SAA. MAbs VSA6 and VSA25 cross-react also with equine SAA and MAb VSA25 with feline SAA.
MAb isotypes:

IgG1 for MAbs SAA1, SAA6, SAA15, VSA6, VSA25
Applications:

All MAbs recognize SAA in ELISA and Western blotting.
Recommended pairs for human SAA sandwich immunoassay (capture - detection):
VSA25 – VSA31
VSA6 – VSA38
(MAbs VSA31 and VSA38 are under Cat.# 4VS4)
Purification:

Chromatography on protein A Sepharose
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Other information:

We recommend to avoid using bovine serum albumin (BSA) as a buffer component or blocking agent for SAA immunoassay. In buffers, BSA can be replaced with 1% casein.

When developing an SAA immunoassay in microtiter plates, it is important to prevent non-specific binding of SAA to the wells of a plate. Plates blocking procedure and antigen dilution buffer should be optimized to ensure that SAA non-specific binding to the plate wells is suppressed. Buffer containing 1% casein and 0.05% Tween 20 is suggested for recommended MAb combinations.
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:

Feline, Canine and Equine Serum Amyloid A (SAA) TechNotes
Human SAA TechNotes
Download datasheet as a PDF file below