Serum amyloid A (SAA), antibody

Cat.# 4SA11

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Inflammation
  • Cat.#

    4SA11

  • MAbs in vitro:

    SAA1cc, SAA15cc

  • MAbs in vivo:

    SAA6, VSA6, VSA25

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with either human SAA (SAA1cc, SAA6, SAA15cc), or synthetic peptides corresponding to the regions 23-29 a.a.r. (VSA25) and 72-86 a.a.r. (VSA6) of human SAA.

  • Specificity:

    Human SAA. All MAbs cross-react with canine SAA. MAbs VSA6 and VSA25 cross-react also with equine SAA and MAb VSA25 with feline SAA.

  • MAb isotypes:

    IgG1 for MAbs SAA1cc, SAA6, SAA15cc, VSA6, VSA25

  • Applications:

    All MAbs recognize SAA in ELISA and Western blotting.
    Recommended pairs for human SAA sandwich immunoassay (capture - detection):
    VSA25 – VSA31cc
    VSA6 – VSA38cc
    (MAbs VSA31cc and VSA38cc are under Cat.# 4VS4)

  • Purification:

    Protein A chromatography

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN)

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Other information:

    We recommend to avoid using bovine serum albumin (BSA) as a buffer component or blocking agent for SAA immunoassay. In buffers, BSA can be replaced with 1% casein.

    When developing an SAA immunoassay in microtiter plates, it is important to prevent non-specific binding of SAA to the wells of a plate. Plates blocking procedure and antigen dilution buffer should be optimized to ensure that SAA non-specific binding to the plate wells is suppressed. Buffer containing 1% casein and 0.05% Tween 20 is suggested for recommended MAb combinations.

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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Serum amyloid A (SAA), antibody

(Catalogue number and MAb: 4SA11-)

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Serum amyloid A (SAA), antibody

(Catalogue number and MAb: 4SA11-)

Evaluation samples available. You can save up to 50%*

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*The usual shipping and handling costs will still apply. The sample opportunity is a one-time evaluation possibility and you can get more details by contacting HyTest customer service.
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