Serum amyloid A (SAA), antibody

Cat.# 4SA11

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Inflammation
  • Cat.#

    4SA11

  • MAbs:

    SAA1, SAA6, SAA15, VSA6, VSA25

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with either human SAA (SAA1, SAA6, SAA15), or synthetic peptides corresponding to the regions 23-29 a.a.r. (VSA25) and 72-86 a.a.r. (VSA6) of human SAA.

  • Specificity:

    Human SAA. All MAbs cross-react with canine SAA. MAbs VSA6 and VSA25 cross-react also with equine SAA and MAb VSA25 with feline SAA.

  • MAb isotypes:

    IgG1 for MAbs SAA1, SAA6, SAA15, VSA6, VSA25

  • Applications:

    All MAbs recognize SAA in ELISA and Western blotting.
    Recommended pairs for human SAA sandwich immunoassay (capture - detection):
    VSA25 – VSA31
    VSA6 – VSA38
    (MAbs VSA31 and VSA38 are under Cat.# 4VS4)

  • Purification:

    Chromatography on protein A Sepharose

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Other information:

    We recommend to avoid using bovine serum albumin (BSA) as a buffer component or blocking agent for SAA immunoassay. In buffers, BSA can be replaced with 1% casein.

    When developing an SAA immunoassay in microtiter plates, it is important to prevent non-specific binding of SAA to the wells of a plate. Plates blocking procedure and antigen dilution buffer should be optimized to ensure that SAA non-specific binding to the plate wells is suppressed. Buffer containing 1% casein and 0.05% Tween 20 is suggested for recommended MAb combinations.

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

  • Additional product information:

    Feline, Canine and Equine Serum Amyloid A (SAA) TechNotes
    Human SAA TechNotes

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Serum amyloid A (SAA), antibody (MAb: )

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