Rat monoclonal antibody produced in bioreactor. Heterohybdridoma clone cell line derived from hybridization of Sp2/0 myeloma cells and spleen cells of rat.
Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.
SAA6, VSA6, VSA25
Mouse monoclonal antibody produced in ascites. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice
Human SAA for A491, A496, SAA1cc, SAA15cc, SAA6
Synthetic peptide corresponding to the region 23-29 a.a.r. of human SAA for VSA25
Synthetic peptide corresponding to the region 72-86 a.a.r. of human SAA for VSA6
IgG1 for A496, SAA1cc, SAA15cc SAA6, VSA6, VSA25
IgG2b for A491
Recommended pairs for human SAA sandwich immunoassay (capture - detection):
A496 – A491
A496 – SAA19cc or SAA19cc – A496
A496 – SAA21cc
(MAbs SAA19cc and SAA21cc available under Cat.# 4VS4)
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
Bovine serum albumin (BSA) is commonly used as a buffer component or blocking agent for immunoassays. Some preparations of BSA might exhibit high background in SAA immunoassays. In case of high background, testing of several different BSA preparations is recommended.
When developing an SAA immunoassay in microtiter plates, non-specific binding of SAA to the wells of a plate might be observed. Plates blocking procedure and antigen dilution buffer might require optimization to ensure that SAA non-specific binding to the plate wells is suppressed. Blocking buffer containing 1% casein and 0.05% Tween 20 is suggested for plate wells blocking.
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
(Catalogue number and MAb: 4SA11-)