Expressed in E.coli
Recombinant SAA consists of 120 amino acids and has a predicted molecular mass of 13580 kDa.
Consists of a polypeptide corresponding to 1-110 a.a.r. of equine SAA and a proprietary 10 a.a.r. affinity tag on the N-terminus.
Can be used as a control in immunoassays.
Purity >95 % (Tricine-SDS-PAGE).
Concentration is determined spectrophotometrically
Affinity and reversed-phase chromatography
Lyophilized from 0.01 M HCl (pH 2.0)
It is recommended to reconstitute this product with 0.01 M HCl (pH 2.0) to 0.1-1 mg/ml concentration.
Lyophilized -20°C (-15 … -30 °C allowed)
Reconstituted -70°C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
To prepare diluted samples of SAA it is recommended to use buffer solutions containing a detergent. The selection of an appropriate detergent and its concentration is essential for the assay performance.
For sandwich immunoassays using monoclonal anti-SAA antibodies SAA19, SAA21 and VSA34 we recommend using 0.01% CHAPS in antigen dilution buffer (e.g. 0.05 M Tris-HCl, pH 8.0, 0.15 M NaCl, 0.01 % CHAPS).
For sandwich immunoassays using monoclonal anti-SAA antibodies VSA6, VSA25, VSA31, VSA38, VSA2 and VSA43 we recommend using 0.05 % Tween 20 in antigen dilution buffer (e.g. 0.02 M TrisHCl, pH 7.5, 0.15 M NaCl, 0.05 % Tween 20).
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.