Recombinant canine serum amyloid A (SAA)

Cat.# 8CS4

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Veterinary
  • Cat.#

    8CS4

  • Source:

    Expressed in E.coli

  • Description:

    Recombinant SAA consists of 121 amino acids and has a predicted molecular mass of 13766 kDa.

    Consists of a polypeptide corresponding to 1-111 a.a.r. of canine SAA and a proprietary 10 a.a.r. affinity tag on the N-terminus.

  • Applications:

    Can be used as a control in immunoassays.

  • Analysis:

    Purity >95 % (Tricine-SDS-PAGE).
    Concentration is determined spectrophotometrically

  • Purification:

    Affinity and reversed-phase chromatography

  • Presentation:

    Lyophilized from 0.01 M HCl (pH 2.0)

    It is recommended to reconstitute this product with 0.01 M HCl (pH 2.0) to 0.1-1 mg/ml concentration.

  • Storage:

    Lyophilized -20°C (-15 … -30 °C allowed)
    Reconstituted -70°C (-65 … -80 °C allowed)

  • Other information:

    Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

    To prepare diluted samples of SAA it is recommended to use buffer solutions containing a detergent. The selection of an appropriate detergent and its concentration is essential for the assay performance.

    For sandwich immunoassays using monoclonal anti-SAA antibodies SAA19, SAA21 and VSA34 we recommend using 0.01% CHAPS in antigen dilution buffer (e.g. 0.05 M Tris-HCl, pH 8.0, 0.15 M NaCl, 0.01 % CHAPS).

    For sandwich immunoassays using monoclonal anti-SAA antibodies VSA6, VSA25, VSA31, VSA38, VSA2, and VSA43 we recommend using 0.05 % Tween 20 in antigen dilution buffer (e.g. 0.02 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.05 % Tween 20).

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

  • References:

    Search references from PubMed

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Recombinant canine serum amyloid A (SAA) (MAb: )

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