Monoclonal mouse anti-serum amyloid A (SAA), animal Cat.#
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VSA34, SAA19, SAA21, SAA11, VSA31, VSA38, VSA2, VSA43
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with either human SAA (SAA19, SAA21, SAA11), or canine SAA (VSA34, VSA31, VSA38), or synthetic peptides derived from the region 79-104 a.a.r. of canine SAA (VSA2, VSA43).
Canine SAA (all MAbs)
IgG1 for MAb VSA2
Recommended pairs for sandwich immunoassay (capture – detection):
Chromatography on protein A Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
|Material safety note:||
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
We recommend to avoid using bovine serum albumin (BSA) as a buffer component or blocking agent for SAA immunoassay. In buffers, BSA can be replaced with 1% casein.
Immunoassay plates blocking with casein is needed to prevent non-specific binding of SAA to the wells of an immunoassay plate.
For pairs SAA19 – VSA34 and SAA21 – VSA34, we recommend to start assay optimization using 0.01% CHAPS in antigen dilution and washing buffers. According to our data, Tween 20 lowered the signal in the wells when it was used in concentration 0.05-0.1% in antigen dilution buffer. When lower concentration of Tween 20 was used (0.005-0.025%), non-specific binding of SAA to the wells of an immunoassay plate was observed.
For pairs VSA2 – VSA38, VSA2 – VSA31 and VSA38 – VSA43, we recommend to use antigen dilution buffer containing 1% casein and 0.05-0.1% Tween 20.
|Additional product information:|
Search serum amyloid A (SAA) references from PubMed
Download datasheet as a PDF file below
- 4VS4_DS.pdf (pdf)