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Monoclonal mouse anti-cardiac troponin I (cTnI) Cat.#

4T21

Data Sheet

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MAbs
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Alternatives in vitro!
Please note that MAbs 4C2, M155, 19C7, 560, 16A12, 16A11 and MF4 are also available as in vitro produced products under Cat.#4T21cc. For immunoassays utilizing of these MAbs, we recommend selecting the in vitro produced product.

Properties
Unit

mg
Cat.#

4T21
MAbs:

8E10, C5, 4C2, 19C7, 16A11, 3C7, P4-14G5, M18, M155, M46, MF4, 16A12, P4-9F6, 228, 625, 458, 267, 596, 84, 415, 581, 10F4, 247, 560, 17F3, p45-10, 916, 909, 820, 810, 801

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cardiac troponin I, native human cardiac troponin complex, or human cardiac troponin I peptides.
Specificity:

None of the MAbs (except M46, 267, 596, and C5) have cross-reaction with skeletal muscle TnI. Cross-reactivity with skeletal TnI is less than 10% for MAbs M46, 267 and 596, and more than 50% for MAb C5. MAb 247 reacts only with free cTnI.

Additional comprehensive information about cross-reactivity with different forms of antigens (phosphorylated, complexed etc.) and antigens from different species (rat, mouse, canine, bovine and porcine) is available.
Please read Cardiac troponin I Booklet here.
MAb isotypes:

IgG1 for MAbs 8E10, 16A11, 3C7, P4-14G5, M18, M155, M46, MF4, P4-9F6, 228, 625, 560, 596, 16A12, 415, 84, 581, 247, 17F3, p45-10, 909, 810, 820
IgG2a for MAbs 4C2, 267, 10F4
IgG2b for MAbs 19C7, C5
IgG3 for MAbs 916, 801
IgM for MAb 458
Applications:

cTnI immunoassay; cTnI immunoprecipitation, immunohistochemistry, immunoaffinity purification.

All MAbs work in Western blotting. For better sensitivity in Western blotting we recommend using MAbs 19C7, 16A11 and MF4.

Recommended pairs for quantitative sandwich immunoassay (capture-detection):
19C7 – 16A11, 19C7 – 560, 625 – 19C7, 560 – 458, 4C2 – 19C7

MAbs 801, 810, 820, 909 and 916 work well as capture MAbs with MAb 19C7 as detection MAb.

Antibody combinations for quantitative sandwich immunoassay:
19C7 + MF4 (mixture for coating) – 7B9 (anti-TnC MAb; Cat. # 4T27)
916 + 560 (mixture for coating) – 19C7 + MF4 (mixture for detection)
801 + 560 (mixture for coating) – 19C7 + MF4 (mixture for detection)
909 + 560 (mixture for coating) – 19C7 + MF4 (mixture for detection)
M18 + 560 (mixture for coating) – 19C7 + MF4 (mixture for detection)
Purification:

Chromatography on protein A Sepharose
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:

Troponin I product information
Cardiac troponin I Booklet
Veterinary product info
Antibodies Produced in vitro
References:

See all Troponin references.
See references where ﾠMonoclonal mouse anti-cardiac troponin I (cTnI) has been used in.
Search references from PubMed.
Download datasheet as a PDF file below