M18cc, 1017cc, 1039cc, 4C2cc, M155cc, 19C7cc, 560cc, 16A11cc, 16A12cc, 8E10cc, MF4cc
P4-14G5, 916, 909, 801, 810, 3C7, 228, 820, 10F4, 247, 17F3, 84, M46, 625, 458, 596, 267, C5, p45-10
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnI, native human cTn complex, or human cTnI peptides.
See epitope specificity in the PDF DataSheet. None of the MAbs (except M46, 267, 596 and C5) have cross-reaction with skeletal muscle TnI. Cross-reactivity with skeletal TnI is less than 10% for MAbs M46, 267 and 596, and more than 50% for MAb C5. MAb 247 reacts only with free cTnI.
IgG1 for M18cc, 1017cc, 1039cc, M155cc, 560cc, 16A11cc, 16A12cc, 8E10cc, MF4cc, P4-14G5, 909, 810, 3C7, 228, 820, 247, 17F3, 84, M46, 625, 596, p45-10
IgG2a for 4C2cc, 10F4, 267
IgG2b for 19C7cc, C5
IgG3 for 916, 801
IgM for 458
cTnI immunoassay; cTnI immunoprecipitation, immunohistochemistry, immunoaffinity purification.
All MAbs work in Western blotting. For better sensitivity in Western blotting we recommend using 19C7cc, 16A11cc and MF4cc.
Pair recommendations for quantitative cTnI sandwich immunoassays: see PDF DataSheet
801, 810, 820, 909 and 916 work well as capture MAbs with 19C7cc as detection MAb.
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
(Catalogue number and MAb: 4T21/4T21cc-)