Recombinant human cardiac troponin I (cTnI) Cat.#

8RTI7

Data Sheet

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Properties
Unit

mg

Cat.#

8RTI7

Source: 

Expressed in E. coli

Protein sequence:

Product of gene TNNI3; 210 a.a.r.

Recombinant human cardiac TnI contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes). For full sequence, see printable -file.

Application: 

Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnI standard

Analysis: 

Purity > 95 % (SDS-PAGE)

Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) - 0.420 (measured at 280 nm)

Immunological  activity: 

Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnI isoform

Purification: 

Immunoaffinity purification followed by ion-exchange chromatography

Presentation: 

Lyophilized from 0.01 M HCl

It is recommended to reconstitute this product with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol), to a concentration close to 1 mg/ml.

Storage: 

Lyophilized -20°C (-15 … -30 °C allowed)
Reconstituted -70°C (-65 … -80 °C allowed)

Other information: 

Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Additional product information:

Troponin I product information
Cardiac troponin I Booklet
Troponin I and Troponin Complex Antibodies

References:

See other Troponin references

Download datasheet as a PDF file below