Recombinant human cardiac troponin I (cTnI) Cat.#
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Expressed in E. coli
Product of gene TNNI3; 210 a.a.r.
Recombinant human cardiac TnI contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes). For full sequence, see printable -file.
Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnI standard
Purity > 95 % (SDS-PAGE)
Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) - 0.420 (measured at 280 nm)
Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnI isoform
Immunoaffinity purification followed by ion-exchange chromatography
Lyophilized from 0.01 M HCl
It is recommended to reconstitute this product with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol), to a concentration close to 1 mg/ml.
Lyophilized -20°C (-15 … -30 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
|Material safety note:||
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
|Additional product information:|
See other Troponin references