Expressed in E. coli
Product of gene TNNI3; 210 a.a.r.
Recombinant human cardiac TnI contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes). For full sequence, see printable -file.
Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnI standard
Purity > 95 % (SDS-PAGE)
Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) - 0.420 (measured at 280 nm)
Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnI isoform
Immunoaffinity purification followed by ion-exchange chromatography
Lyophilized from 0.01 M HCl
It is recommended to reconstitute this product with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol), to a concentration close to 1 mg/ml.
Lyophilized -20°C (-15 … -30 °C allowed)
Reconstituted -70°C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.