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Recombinant human cardiac troponin I (cTnI) Cat.#

8RTI7

Data Sheet

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Properties
Cat.#

8RTI7

Source: 

Expressed in E. coli

Protein sequence:

Product of gene TNNI3; 210 a.a.r.

MADGSSDAAR EPRPAPAPIR RRSSNYRAYA TEPHAKKKSK ISASRKLQLK TLLLQIAKQE LEREAEERRG EKGRALSTRC QPLELAGLGF AELQDLCRQL HARVDKVDEE RYDIEAKVTK NITEIADLTQ KIFDLRGKFK RPTLRRVRIS ADAMMQALLG ARAKESLDLR AHLKQVKKED TEKENREVGD WRKNIDALSG MEGRKKKFES

Recombinant human cardiac TnI contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes). For full sequence, see printable -file.

Application: 

Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnI standard

Analysis: 

Purity > 95 % (SDS-PAGE)

Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) - 0.420 (measured at 280 nm)

Immunological  activity: 

Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnI isoform

Purification: 

Immunoaffinity purification followed by ion-exchange chromatography

Presentation: 

Lyophilized from 0.01 M HCl

It is recommended to reconstitute this product with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol), to a concentration close to 1 mg/ml.

Storage: 

Lyophilized -20°C (-15 … -30 °C allowed)
Reconstituted -70°C (-65 … -80 °C allowed)

Other information: 

Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Additional product information:

Troponin I product information
Cardiac troponin I Booklet

References:

See other Troponin references

Download datasheet as a PDF file below