Recombinant human cardiac Troponin I (cTnI)

Cat.# 8RTI7

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Cardiac markers
  • Cat.#

    8RTI7

  • Source: 

    Expressed in E. coli

  • Protein sequence:

    Product of gene TNNI3; 210 a.a.r.

    Recombinant human cardiac TnI contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes). For full sequence, see printable -file.

  • Application: 

    Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnI standard

  • Analysis: 

    Purity > 95 % (SDS-PAGE)

    Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) - 0.420 (measured at 280 nm)

  • Immunological  activity: 

    Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnI isoform

  • Purification: 

    Immunoaffinity purification followed by ion-exchange chromatography

  • Presentation: 

    Lyophilized from 0.01 M HCl

    It is recommended to reconstitute this product with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol), to a concentration close to 1 mg/ml.

  • Storage: 

    Lyophilized -20°C (-15 … -30 °C allowed)
    Reconstituted -70°C (-65 … -80 °C allowed)

  • Other information: 

    Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

  • References:
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Recombinant human cardiac Troponin I (cTnI) (MAb: )

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