-
Cat.#
8RTI7
-
Source:
Expressed in E. coli
-
Protein sequence:
Product of gene TNNI3; 210 a.a.r. Recombinant human cardiac TnI contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes). For full sequence, see printable -file.
-
Application:
Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnI standard
-
Analysis:
Purity > 95 % (SDS-PAGE) Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) - 0.420 (measured at 280 nm)
-
Immunological activity:
Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnI isoform
-
Purification:
Immunoaffinity purification followed by ion-exchange chromatography
-
Presentation:
Lyophilized from 0.01 M HCl It is recommended to reconstitute this product with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol), to a concentration close to 1 mg/ml.
-
Storage:
Lyophilized -20°C (-15 … -30 °C allowed)Reconstituted -70°C (-65 … -80 °C allowed)
-
Other information:
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
-
Material safety note:
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
