Troponin I Cardiac - Human

Cat.# 8T53

This data sheet is provided for information only. After adding the product to the cart by clicking the button below you will see if there is a volume discount available.

Sign up or login to see prices and to request offers

Price:
-- / 0.1 mg
Login for price
* All prices excluding VAT
Request an offer Sample Request

Questions? Schedule online meeting

Cardiac markers
  • Cat.#

    8T53

  • Source:

    Human heart tissue.

    Blood sample from the tissue donors was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.

  • Applications:

    TnI is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium sensitivity to striated muscle actomyosin ATPase activity.

    TnI exists in three isoforms, one for slow-twitch skeletal muscle, one for fast twitch skeletal muscle and one for cardiac muscle. The amino acid sequences of two skeletal (skTnI) and one cTnI forms how 40% dissimilarity. Furthermore, the human cTnI has additional amino acid residues on its N-terminal region that are not present on the skeletal form, making this protein a promising analyte for indicating cardiac specificity. cTnI follows similar release pattern as CK-MB within the first 15 hours of myocardial infarction (MI), but remains elevated in serum up to 6 days. This observation and the unique amino sequence of cTnI make it an important diagnostic marker of MI.

    TnI is suitable for use as a standard in immunoassay for early detection of MI, immunogen for antiserum production and tracer for iodination.

  • Analysis:

    Purity > 98 % (SDS-PAGE).

    Immunological activity confirmed by reaction with monoclonal antibody that is specific for the troponin I cardiac isoform in immunoblotting.

    TnI concentration was determined spectrophotometrically using A (0.1 %, 280 nm, 1 cm) equal to 0.42. This coefficient was calculated from the amino composition of human cTnI (FEBS Lett, 270, 57-61).

  • Presentation:

    Lyophilized from 0.01 M HCl.

    It is recommended to reconstitute this product with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol) to a concentration close to 1 mg/ml.

    Solubility is limited at neutral pH and physiological salt concentration.

  • Storage:

    Lyophilized -20°C (-15 … -30 °C allowed)
    Reconstituted -70°C (-65 … -80 °C allowed)

  • Other information:

    Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

×

Bulk Price Quotation

You can request quotations by filling in the desired amount and your information. Please note that if you register or login you can see the prices immediately without waiting for quotes. You can also request a sample here.

Troponin I Cardiac - Human

(Catalogue number and MAb: 8T53-)

Information

×

Sample Request

Troponin I Cardiac - Human

(Catalogue number and MAb: 8T53-)

Evaluation samples available. You can save up to 50%*

Information

*The usual shipping and handling costs will still apply. The sample opportunity is a one-time evaluation possibility and you can get more details by contacting HyTest customer service.
×

Schedule Online Meeting

Please fill in the form below and we will contact you by email and schedule a meeting.

Information

Sign up to our newsletter

And be informed about new products and scientific findings

Thank you for your subscription!