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Cat.#
4T21/4T21cc
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MAbs in vitro (Cat.# 4T21cc):
Y101, M18cc, 1017cc, 1039cc, 4C2cc, M155cc, 19C7cc, 560cc, 16A11cc, 16A12cc, 8E10cc, MF4cc Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.
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MAbs in vivo (Cat. 4T21):
P4-14G5, 916, 909, 801, 810, 3C7, 228, 820, 10F4, 247, 17F3, 84, M46, 625, 458, 596, 267, C5, p45-10 Mouse monoclonal antibody produced in ascites. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.
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Immunogens:
Free human cTnI for 16A11cc, 16A12cc, 19C7cc, 4C2cc, 8E10cc, M155cc, M18cc, MF4cc, 10F4, 17F3, 458, 84, C5, M46Native human cTn complex for 560cc, 228, 247, 625Native human cTn complex and human cTnI peptides for 810, 820Native human cTn complex and artificial human cTn complex for 801Free human cTnI and native human cTn complex 267, 3C7, 596,Free human cTnI and human cTnI peptides for 1017cc, 1039cc, 909, 916Free human cTnI, native human Tn complex and human cTnI peptides for Y101Human cTnI peptides for P4-14G5, p45-10
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Specificity:
Epitope specificity: None of the MAbs (except M46, 267, 596 and C5) have cross-reaction with skeletal muscle TnI. Cross-reactivity with skeletal TnI is less than 10% for M46, 267 and 596, and more than 50% for C5. 247 reacts only with free cTnI.
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MAb isotypes:
IgG1 for Y101, M18cc, 1017cc, 1039cc, M155cc, 560cc, 16A11cc, 16A12cc, 8E10cc, MF4cc, P4-14G5, 909, 810, 3C7, 228, 820, 247, 17F3, 84, M46, 625, 596, p45-10.IgG2a for 4C2cc, 10F4, 267IgG2b for 19C7cc, C5IgG3 for 916, 801IgM for 458
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Applications:
cTnI immunoassay; cTnI immunoprecipitation, immunohistochemistry, immunoaffinity purification.MAbs work in Western blotting. For better sensitivity in Western blotting we recommend using 19C7cc, 16A11cc and MF4cc.Pair recommendations for quantitative cTnI sandwich immunoassays: 801, 810, 820, 909 and 916 work well as capture MAbs with 19C7cc as detection MAb.
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Purification:
Protein A chromatography
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Presentation:
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
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Storage:
+4 °C (+2 … +8 °C allowed)
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Material safety note:
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material. Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
