Monoclonal mouse anti-cardiac Troponin T (cTnT) Cat.#

4T19

Data Sheet

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MAbs
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Alternatives in vitro!
Please note that MAb 1C11 and 1F11 are also available as in vitro produced product under Cat.#4T19cc. For immunoassays utilizing of this MAb, we recommend selecting the in vitro produced product.

Properties
Unit

mg
Cat.#

4T19
MAbs:

9G6, 7F4, 7G7, 2F3, 1A11, 1C11, 1F11, 7E7

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnT (MAb 2F3, 7G7, 1A11, 9G6, 1C11, 1F11, 7F4) or human Tn complex (MAb 7E7).
Specificity:

MAbs 2F3, 7F4, 7G7, 1A11, 9G6, 1C11 and 1F11 have no cross-reaction with skeletal TnT. Cross-reactivity of MAb 7E7 with cardiac TnI is 5 %.
MAb isotypes:

IgG1 for MAbs 9G6, 1C11, 7G7 and 7E7
IgG2b for MAbs 2F3, 1A11, 7F4 and 1F11
Applications:

TnT immunoassay and TnT immunoaffinity purification. It is possible to use following pairs of antibodies for sandwich immunoassay (capture-detection): 1C11-9G6, 1C11-7F4, 7G7-9G6, 7G7-1C11, 7G7-2F3, 1C11-7E7

Best capture antibodies for BIAcore are 1C11, 9G6, 7G7.
Purification:

Protein A Sepharose chromatography.
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:

Troponin booklet
Troponin T Antibodies
Veterinary product info
References:

See other cardiac and blood coagulation products.
See all Troponin references here and here.
Search other Troponin ﾠT references from PubMed.
Download datasheet as a PDF file below