Alternatives in vitro!
Please note that MAb 1C11 and 1F11 are also available as in vitro produced product under Cat.#4T19cc. For immunoassays utilizing of this MAb, we recommend selecting the in vitro produced product.
9G6, 7F4, 7G7, 2F3, 1A11, 1C11, 1F11, 7E7
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnT (MAb 2F3, 7G7, 1A11, 9G6, 1C11, 1F11, 7F4) or human Tn complex (MAb 7E7).
MAbs 2F3, 7F4, 7G7, 1A11, 9G6, 1C11 and 1F11 have no cross-reaction with skeletal TnT. Cross-reactivity of MAb 7E7 with cardiac TnI is 5 %.
IgG1 for MAbs 9G6, 1C11, 7G7 and 7E7
IgG2b for MAbs 2F3, 1A11, 7F4 and 1F11
TnT immunoassay and TnT immunoaffinity purification. It is possible to use following pairs of antibodies for sandwich immunoassay (capture-detection): 1C11-9G6, 1C11-7F4, 7G7-9G6, 7G7-1C11, 7G7-2F3, 1C11-7E7
Best capture antibodies for BIAcore are 1C11, 9G6, 7G7.
Protein A Sepharose chromatography.
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.