4T19 / 4T19cc
300cc, 329cc, 406cc, 1C11cc, 1F11cc
9G6, 7F4, 7G7, 2F3, 1A11, 7E7
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic human TnT peptide a.a.r. 119-138 conjugated with carrier protein (300cc and 329cc), or synthetic human TnT peptide a.a.r. 106-183 conjugated with carrier protein (406cc), free human cTnT (2F3, 7G7, 1A11, 9G6, 1C11cc, 1F11cc, 7F4) or human Tn complex (7E7).
406cc, 1C11cc, 1F11cc, 2F3, 7F4, 7G7, 1A11 and 9G6 have no cross-reaction with skeletal TnT.Cross-reactivity of 300cc and 329cc with skeletal TnT is less than 1%. Cross-reactivity of 7E7 with cardiac TnI is 5 %.
IgG1 for 300cc, 329cc, 1C11cc, 9G6, 7G7, 7E7
IgG2a for 406cc
IgG2b for 1F11cc 2F3, 1A11, 7F4
TnT immunoassay. Recommended pairs for sandwich immunoassay (capture – detection):
406cc – 300cc
329cc – 406cc
1C11cc and 1F11cc are recommended for Western blotting.
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.