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Recombinant human cardiac troponin T (cTnT) Cat.#

8RTT5

Data Sheet

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Properties
Cat.#

8RTT5

Synonym names

Human cardiac troponin T adult isoform, human cardiac troponin T isoform #6 or TnT3

Source:

Expressed in E. coli

Protein sequence:

Product of gene TNNT2; 288 a.a.r.

MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN ММHFGGYIQK QAQTERKSGK RQTEREKKKK ILAERRKVLA IDHLNEDQLR EKAKELWQSI YNLEAEKFDL QEKFKQQKYE INVLRNRIND NQKVSKTRGK AKVTGRWK

Recombinant human cardiac TnT contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes).

Application:

Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnT standard

Analysis:

Purity > 95 % (SDS-PAGE)

Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) - 0.490 (measured at 280 nm)

Immunologicalᅠ activity:ᅠ

Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnT isoform

Purification:

Immunoaffinity purification followed by ion-exchange chromatography

Presentation:

Lyophilized from 0.01 M HCl

It is recommended to reconstitute this product with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol), to a concentration close to 1 mg/ml.

Storage:

Lyophilized -20°C (-15 … -30 °C allowed)
Reconstituted -70 °C (-65 … -80 °C allowed)

Other information:

Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Additional product information:

Troponin booklet

References:

See all Troponin references

Download datasheet as a PDF file below
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