Expressed in a mammalian cell line
Synthetic DNA fragment encoding a full-sized (1-2768 a.a.r.) subunit of human thyroglobulin including signal peptide was used according UniProtKB P01266 accession number.
Contains C-terminal GlyAlaProGly4SerHis10-tag.
Theoretical MW: 304640 Da including the C-terminal tag
Theoretical pI: 5.54
To be used as an antigen in the assays for the detection of human thyroglobulin-specific autoantibodies.
Calibrator or standard in immunoassays.
Concentration is determined spectrophotometrically at A280. Abs 1 mg/ml = 1.14.
Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to human thyroglobulin.
Metal affinity chromatography
Lyophilized from PBS, pH 7.4, 5.4 % sucrose.
It is recommended to reconstitute this product with deionized water to its initial concentration.
Lyophilized at -20 °C (-15 … -30 °C allowed), reconstituted at -70 °C (-65 … -80 °C allowed).
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
(Catalogue number and MAb: 8RTG4)