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Cat.#
2TG12cc
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Recombinant MAbs:
TG51, TG61, TG64, TG66Recombinant chimeric antibody expressed in a mammalian cell line. Composed of original wild type variable domains of rat derived MAb and human IgG1 constant domains.TG12 Recombinant monoclonal antibody expressed in a mammalian cell line. Full-size IgG sequence derived from rabbit B cells
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MAbs in vitro:
TG08, TG14, TG16, TG23, TG33, TG36, TG37, TG46, TG47, 5E6cc, 5F9ccMouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization ofSp2/0 myeloma cells with spleen cells of Balb/c mice.
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Immunogen:
Thyroglobulin from human thyroid gland
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Specificity:
Human thyroglobulin. TG12, TG14, TG16, TG23, TG33, TG36, TG37, TG46, TG47, TG64, 5E6cc, 5F9cc exhibit no or low interference with thyroglobulin autoantibodies.TG08, TG51, TG61, TG66 compete with thyroglobulin autoantibodies (exhibit high interference).5F9cc cross-reacts with bovine thyroglobulin
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MAb isotypes:
gG for TG12IgG1 for TG08, TG14, TG16, TG23, TG36, TG37, TG47, TG51, TG61, TG64, TG66IgG2a for TG46, 5F9ccIgG2b for 5E6cc, TG33
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Applications:
Detection of human thyroglobulin in immunoassays. Recommended pairs for sandwich immunoassay: Capture Detection TG47 TG16 TG47 TG64 TG16 TG12 TG16 TG33 TG14 TG23 TG64 TG23 TG64 TG16 TG23 TG12 5F9cc 5E6cc TG37 TG16 TG46 TG12 TG64 TG46 TG37 TG64 TG08, TG51, and TG61 are intended for developing immunoassays for the quantitative determination of thyroglobulin autoantibodies, as these MAbs target thyroglobulin epitopes recognized by autoantibodies. TG08, TG51, or TG61 can be used individually in competitive immunoassays. In addition, TG08 or TG61 can be combined with the supplemental antibody TG66 in competitive formats; the recommended primary-to-supplemental antibody ratio is 3:1.MAbs TG08, TG12, TG14, TG16, TG23, TG36, TG37, TG46, TG47, TG51, TG61, TG64, 5E6cc and5F9cc work in Western blotting.
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Purification:
Protein A chromatography
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Presentation:
50 mM sodium citrate, 150 mM NaCl, pH 6.0, 0.09 % sodium azide (NaN3) for TG33, TG36, TG37, TG46, TG47PBS, pH 7.4, 0.09 % sodium azide (NaN3) for TG08, TG12, TG14, TG16, TG23, TG51, TG61, TG64, TG66, 5E6cc, 5F9cc
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Storage:
+4 °C (+2 … +8 °C allowed)
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Material safety note:
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material. Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
