Expressed in a mammalian cell line
The extracellular domain of human angiotensin-converting enzyme 2, containing at the C-terminus the Fc fragment of human immunoglobulin G (IgG1); Synthetic DNA fragment encoding amino acids 1-740 of human angiotensin-converting enzyme 2 (Accession number UniProtKB Q9BYF1; ACE2_HUMAN) was used for recombinant protein expression.
Theoretical MW: 108045.42 Da
Theoretical pI: 5.53
SARS-CoV-2 Spike RBD (receptor-binding domain) binding assays
Concentration is determined by absorbance at 280 nm. Abs 1 mg/ml = 1.85
Protein A chromatography
Lyophilized from 50 mM Tris-HCl, 150 mM NaCl, 1 % trehalose, pH 7.5
Lyophilized at -20 °C (-15 … -30 °C allowed)
Reconstituted at -70 °C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product.
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
(Catalogue number and MAb: 8AE5)