3A1, 1G4, 1D6
Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human proinsulin.
MAbs 3A1 and 1G4 are directed to different antigenic determinants. MAb 3A1 reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule.
MAb 1D6 reacts with different epitopes on human proinsulin molecule; both of each are generated by the interactions between insulin and C-peptide parts of prohormone.
MAbs 3A1, 1G4 and 1D6 do not cross-react with human, bovine and porcine insulin, bovine and porcine proinsulin, and human C-peptide.
IgG1 for MAbs 3A1, 1G4, 1D6
Detection of human proinsulin in two-site enzyme immunoassay.
Recommended pairs for sandwich immunoassay (coating - conjugate):
MAbs 3A1 and 1G4 can be used in immunohistochemistry on frozen sections.
Specific immunosorbtional removal of proinsulin molecules (with immobilized 1D6 MAb) from serum samples before the measurement of insulinic C-peptide.
Chromatography on protein A Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.