Monoclonal mouse anti-human proinsulin Cat.#

2P9

Data Sheet

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MAbs
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Properties
Unit

mg
Cat.#

2P9
MAbs:

3A1, 1G4, 1D6

Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human proinsulin.
Specificity:

MAbs 3A1 and 1G4 are directed to different antigenic determinants. MAb 3A1 reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule.

MAb 1D6 reacts with different epitopes on human proinsulin molecule; both of each are generated by the interactions between insulin and C-peptide parts of prohormone.

MAbs 3A1, 1G4 and 1D6 do not cross-react with human, bovine and porcine insulin, bovine and porcine proinsulin, and human C-peptide.
MAb isotypes:

IgG1 for MAbs 3A1, 1G4, 1D6
Applications:

Detection of human proinsulin in two-site enzyme immunoassay.

Recommended pairs for sandwich immunoassay (coating - conjugate):

3A1 - 7F8 (Cat.# 2I1)
1G4 - mixture of D6C4cc and D3E7cc (Cat.# 2IP10cc)
1D6 - 7F8, 8E2 (Cat.# 2I1), D6C4cc or D3E7cc (Cat.# 2IP10cc) (Could be used for direct coating of prohormone in serum samples).

MAbs 3A1 and 1G4 can be used in immunohistochemistry on frozen sections.

Specific immunosorbtional removal of proinsulin molecules (with immobilized 1D6 MAb) from serum samples before the measurement of insulinic C-peptide.
Purification:

Chromatography on protein A Sepharose
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:

Rat C-peptide and proinsulin TechNotes
Diabetes marker products
References:

See other references.
See other Metabolic syndrome products.
Search Proinsulin references from PubMed.
Download datasheet as a PDF file below
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