Alternative in vitro!
Please note that MAb 10E1 and 10E2 are also available as in vitro produced product under Cat.#4P41cc. For immunoassays utilizing this MAb, we recommend selecting the in vitro produced product.
10E1, 10E2, 5H9, 4G11, 3C8, 10H9, 11E4, 7A6, PAPP52
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human PAPP-A/proMBP (heterotetrameric PAPP-A, htPAPP-A) complex or purified human atherosclerotic tissue form of PAPP-A (MAb PAPP52).
Human PAPP-A antigen purified from placental blood (heterotetrameric complex, consisting of PAPP-A subunits and proMBP subunits). MAbs 11E4 and 5H9 are specific to proMBP subunit.
IgG1 for MAb PAPP52
IgG2a for MAbs 4G11, 3C8, 10H9, 7A6
IgG2b for MAbs 5H9, 11E4, 10E1, 10E2
htPAPP-A and dPAPP-A (PAPP-A dimer) immunoassays and immunoprecipitation.
Recommended pairs for detection of total PAPP-A (htPAPP-A and/or dPAPP-A) (capture - detection):
10E2 - 10E1
4G11 - 3C8
4G11 - 10H9
10E1 - 7A6
Recommended pairs for detection of human htPAPP-A antigen (capture - detection):
10E2 - 5H9
5H9 - 10E2
5H9 - 7A6
10E1 - 11E4
Please see Cat.# 4PD4 Data Sheet for recommended pairs for specific dPAPP-A antigen detection.
PAPP-A immunodetection in Western blotting. MAbs 4G11 and 10E1 recognize htPAPP-A, consisting of PAPP-A subunits and proMBP subunits, in non-reducing conditions.
Chromatography on protein A Sepharose.
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.