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Monoclonal mouse anti-human pregnancy-associated plasma protein A (PAPP-A) Cat.#

4P41

Data Sheet

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Properties
Unit

mg

Cat.#

4P41

MAbs:

10E1, 10E2, 5H9, 4G11, 3C8, 10H9, 11E4, 7A6, PAPP52

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human PAPP-A/proMBP (heterotetrameric PAPP-A, htPAPP-A) complex or purified human atherosclerotic tissue form of PAPP-A (MAb PAPP52).

Specificity:

Human PAPP-A antigen purified from placental blood (heterotetrameric complex, consisting of PAPP-A subunits and proMBP subunits). MAbs 11E4 and 5H9 are specific to proMBP subunit.

MAb isotypes:

IgG1 for MAb PAPP52
IgG2a for MAbs 4G11, 3C8, 10H9, 7A6
IgG2b for MAbs 5H9, 11E4, 10E1, 10E2

Applications:

htPAPP-A and dPAPP-A (PAPP-A dimer) immunoassays and immunoprecipitation.

Recommended pairs for detection of total PAPP-A (htPAPP-A and/or dPAPP-A) (capture - detection):

10E2 - 10E1
4G11 - 3C8
4G11 - 10H9
10E1 - 7A6

Recommended pairs for detection of human htPAPP-A antigen (capture  - detection):

10E2 - 5H9
5H9 - 10E2
5H9 - 7A6
10E1 - 11E4

Please see Cat.# 4PD4 Data Sheet for recommended pairs for specific dPAPP-A antigen detection.

PAPP-A immunodetection in Western blotting. MAbs 4G11 and 10E1 recognize htPAPP-A, consisting of PAPP-A subunits and proMBP subunits, in non-reducing conditions.

Purification:

Chromatography on protein A Sepharose.

Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN)

Storage:

+4 °C (+2 … +8 °C allowed)

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Additional product information:

Pregnancy Associated Plasma Protein A (PAPP-A) TechNotes

References:

See other fertility products.
See all PAPP-A References.
Search PAPP-A references from PubMed.

Download datasheet as a PDF file below