Neuron-specific Enolase (NSE)

Cat.# 8NS3

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Neuroscience
  • Cat.#

    8NS3

  • Source:

    Post mortal human brain.

    Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies, and syphilis.

  • Analysis:

    Purity > 95 % (SDS-PAGE).

  • Activity:

    Specific activity is determined for each lot at 25 °C in 45 mM imidazole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4; 1 mM 2-PG. One unit is defined as the amount of NSE converted 1 mol of 2-phospho-D-glycerate per min at 25 °C (J.Biochem. 87, 1587-1594, 1980). Subunit composition is gamma-gamma.

  • Immunological activity:

    Tested in ELISA and immunoblotting with anti-NSE antibodies.

  • Presentation:

    0.1 M Tris-HCl, 5 mM MgSO4, 0.25 M KCl, pH 5.1.

  • Purification:

    Ion-exchange chromatography and gel-filtration.

  • Storage:

    -70 °C (-65 … -80 °C allowed)

  • Other information:

    Avoid repeated freezing and thawing.

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

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Neuron-specific Enolase (NSE) (MAb: )

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