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Neuron-specific enolase (NSE) Cat.#

8NS3

Data Sheet

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Properties
Cat.#

8NS3

Source:

Post mortal human brain.

Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies, and syphilis.

Analysis:

Purity > 95 % (SDS-PAGE).

Purification:

Ion-exchange chromatography and gel-filtration.

Activity:

Specific activity is determined for each lot at 25 °C in 45 mM imidazole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4; 1 mM 2-PG. One unit is defined as the amount of NSE converted 1 mol of 2-phospho-D-glycerate per min at 25 °C (J.Biochem. 87, 1587-1594, 1980). Subunit composition is gamma-gamma.

Immunological activity:

Tested in ELISA and immunoblotting with anti-NSE antibodies.

Presentation:

0.1 M Tris-HCl, 5 mM MgSO4, 0.25 M KCl, pH 5.1.

Storage:

-20 °C (-15 … -30 °C allowed)

Other information:

Avoid repeated freezing and thawing.

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Download datasheet as a PDF file below