Post mortal human brain.
Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies, and syphilis.
Purity > 95 % (SDS-PAGE).
Specific activity is determined for each lot at 25 °C in 45 mM imidazole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4; 1 mM 2-PG. One unit is defined as the amount of NSE converted 1 mol of 2-phospho-D-glycerate per min at 25 °C (J.Biochem. 87, 1587-1594, 1980). Subunit composition is gamma-gamma.
Tested in ELISA and immunoblotting with anti-NSE antibodies.
0.1 M Tris-HCl, 5 mM MgSO4, 0.25 M KCl, pH 5.1.
Ion-exchange chromatography and gel-filtration.
-70 °C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing.
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.