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Neuron-specific enolase (NSE) Cat.#

8NS3

Data Sheet

This data sheet is provided for information only. After adding the product to the cart by clicking the button below you will see if there is a volume discount available.

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Properties
Cat.#

8NS3
Source:

Post mortal human brain.

Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies, and syphilis.
Analysis:

Purity > 95 % (SDS-PAGE).
Purification:

Ion-exchange chromatography and gel-filtration.
Activity:

Specific activity is determined for each lot at 25 °C in 45 mM imidazole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4; 1 mM 2-PG. One unit is defined as the amount of NSE converted 1 mol of 2-phospho-D-glycerate per min at 25 °C (J.Biochem. 87, 1587-1594, 1980). Subunit composition is gamma-gamma.
Immunological activity:

Tested in ELISA and immunoblotting with anti-NSE antibodies.
Presentation:

0.1 M Tris-HCl, 5 mM MgSO4, 0.25 M KCl, pH 5.1.
Storage:

-70 °C (-65 … -80 °C allowed)
Other information:

Avoid repeated freezing and thawing.
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Download datasheet as a PDF file below