5G10, 5E2, 1C1, H11
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified NSE from human brain.
IgG2a for MAbs 5E2, 1C1, H11
IgG2b for MAb 5G10
MAbs are working in ELISA, Western blotting and in immunohistochemistry on formalin-fixed and paraffin-embedded sections.
Recommended pairs for sandwich immunoassay (capture-detection):
5G10 – 1C1
5E2 – 1C1
1C1 – 5E2
Protein G chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
(Catalogue number and MAb: 4N6-)