5G10, 5E2, 5A4, 1C1, H11, 2F1
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified NSE from human brain.
IgG2a for MAbs 5E2, 1C1, 5A4, H11, 2F1
IgG2b for MAb 5G10
MAbs 5G10, 5A4 and 5E2 react with 45 kD band corresponding to NSE in immunoblotting of human brain extracts. MAbs may be used for immunohistochemistry on formalin-fixed and paraffinembedded sections.
All MAbs work in ELISA.
Recommended pairs for sandwich immunoassay (capture - detection):
5G10 - 1C1
5E2 - 1C1
1C1 - 5E2
5A4 - 1C1
H11 - 2F1
Chromatography on protein G Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.