Neuron-Specific Enolase, Human, antibody

Cat.# 4N6

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Neuroscience
  • Cat.#

    4N6

  • MAbs:

    5G10, 5E2, 5A4, 1C1, H11, 2F1

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified NSE from human brain.

  • Specificity:

    Human NSE

  • MAb isotypes:

    IgG2a for MAbs 5E2, 1C1, 5A4, H11, 2F1
    IgG2b for MAb 5G10

  • Applications:

    MAbs 5G10, 5A4 and 5E2 react with 45 kD band corresponding to NSE in immunoblotting of human brain extracts. MAbs may be used for immunohistochemistry on formalin-fixed and paraffinembedded sections.

    All MAbs work in ELISA.

    Recommended pairs for sandwich immunoassay (capture - detection):

    5G10 - 1C1
    5E2 - 1C1
    1C1 - 5E2
    5A4 - 1C1 
    H11 - 2F1

  • Purification:

    Chromatography on protein G Sepharose

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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Neuron-Specific Enolase, Human, antibody (MAb: )

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