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Monoclonal mouse anti-neuron specific enolase (NSE) Cat.#

4N6

Data Sheet

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MAbs
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Properties
Unit

mg
Cat.#

4N6
MAbs:

5G10, 5E2, 5A4, 1C1, H11, 2F1

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified NSE from human brain.
Specificity:

Human NSE
MAb isotypes:

IgG2a for MAbs 5E2, 1C1, 5A4, H11, 2F1
IgG2b for MAb 5G10
Applications:

MAbs 5G10, 5A4 and 5E2 react with 45 kD band corresponding to NSE in immunoblotting of human brain extracts. MAbs may be used for immunohistochemistry on formalin-fixed and paraffinembedded sections.

All MAbs work in ELISA.

Recommended pairs for sandwich immunoassay (capture - detection):

5G10 - 1C1
5E2 - 1C1
1C1 - 5E2
5A4 - 1C1 
H11 - 2F1
Purification:

Chromatography on protein G Sepharose
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Download datasheet as a PDF file below