CM1, M9E7, M4H2, M8C9, M2F1, M2C5, M2E104
Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with:
a) high molecular weight (>300 kDa) glycoprotein from human milk-fat globule molecule (MAbs M2F1, M2E104).
b) recombinant (E. coli) VNTR20 (tandem repeat in MUC1 core) polypeptide (MAbs CM1, M9E7, M4H2, M8C9)
MAbs CM1 , M9E7, M8C9:
MAbs bind with high efficiency with different regions of GVTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule.
They react with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC1 prepared from tumor fluids (T47D cells) or as a result of chemical treatment (NaJO4) of human milk MUC1.
These MAbs do not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific MAbs and belong to MAb cluster 1-4 according to ISOMB TD-4 classification.
No cross-reactivity with egg white avidin.
MAbs M4H2, M2F1, M2C5, M2E104:
MAbs bind with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide-carbohydrate compositions.
MAb M4H2 reacts with VNTR20 recombinant unglycosylated fragment of MUC1 protein, with monomeric VNTR MUC1 peptide, with deglycosylated and underglycosylated natural MUC1 protein.
MAbs M2F1, M2C5, M2E104 efficiently recognize underglycosylated and natural MUC1 protein isolated from human milk by affinity chromatography. All these MAbs belong to MAb clusters 6 and 7 according to ISOMB TD-4 classification. Non crossreactive with normal serum proteins and human tissues of non-epithelial origin.
IgG for MAbs M9E7, M4H2, M8C9, M2F1, M2C5, M2E104
IgG1 for MAb CM1
Immunohistochemical and immunofluorescent detection of neoplastic breast epithelium. Antibodies are working on frozen tissue sections. Construction of immunometric assay for MUC determination.
MAbs M9E7, M4H2, M8C9 can be used for construction of highly effective ELISA tests for detection and measurements of unglycosylated and underglycosylated tumor-associated MUC1 mucins in human serum. They may be used as solid-phase reagents for MUC1 isoforms capture
(a) in conjunction with the same MAbs enzyme-conjugated versions (detection of unglycosylated MUC1), or
(b) with enzyme-conjugated MAbs M2C5 and M2E104 specific to carbohydrate portions of MUC1 (detection of underglycosylated MUC1).
MAbs M4H2, M2F1, M2C5, M2E104 can be used for immunohistochemical staining of paraffin and frozen sections of breast, lung and ovarian tumor tissues. They may be used for staining of paraffin-embedded tissue sections fixed in neutral-buffered formalin and is compatible with commonly used histological fixatives. Revealed MUC1 epitopes are abundantly present as on the surface of growing tumor cell lines, so as on the surface and into the cytoplasm of malignant cells of epithelial origin. These MAbs can be used in the form of enzyme conjugates for development in ELISA sandwich assays of under-glycosylated tumor-associated MUC1 mucins in human serum. They may be used in conjunction with MAbs M9E7, M4H2, M8C9 specific to unglycosylated VNTR region of MUC1 as capture reagents.
Chromatography on protein A Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.