Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human IGFBP-5 recombinant fragment expressed in E. coli corresponding to amino acid residues Lys164-Glu272 (MAb IBPF87) or synthetic peptide from human IGFBP-5 corresponding to amino acid residues Cys100-Glu120 (MAb IBPF12)
IgG1 for MAb IBPF12
IgG2a for MAb IBPF87
All MAbs work in direct ELISA and Western Blotting.
Recommended pair for IGFBP-5 immunodetection in sandwich immunoassay (capture-detection):
IBPF12 – IBPF87
MAb IBPF87 can be used for immunoaffinity purification.
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
Some applications in which these products may be used are covered by patents issued and applicable in certain countries. Because purchase of these products does not include a license to perform any patented application, users of these products may be required to obtain a patent license, depending on the particular application and country in which the product is used.
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.