C10F5, AE3, AB5
Hybridoma clones have been derived from hybridization of X63.Ag8.653 myeloma cells with spleen cells of Balb/c mice immunized with recombinant human interferon ⍺-A.
MAb C10F5 recognizes common epitopes of ⍺-A, -F, -D, -I1, -P human interferon subtypes. MAb C10F5 reacts with different antigenic epitope, than NK-2.YOK MAb (Celltech).
IgG1 for MAbs C10F5, AB5
IgG2a for MAbs AE3
MAb C10F5 can be used for the detection of recombinant ⍺-A, -F, - D, I1, -P interferon subtypes in two-site immunoassay and in affinity purification of recombinant interferons of ⍺-A, -F, -D.
MAbs AE3 and AB5 can be used in ELISA and in Western blotting. The recommended pair for sandwich immunoassay (capture - detection): AE3 - AB5 (detection limit 5 pg/ml).
Chromatography on protein A Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.