8G47
Expressed in E. coli
Synthetic DNA fragment encoding amino acids 60-383 of human GFAP (Accession number UniProtKB P14136, GFAP_HUMAN) was used for recombinant protein expression.
Theoretical molecular weight 37 906 Da
Theoretical pI 4.95
Calibrator and standard preparation
Purity >90% (SDS-PAGE in reducing conditions)
Concentration is determined spectrophotometrically at A280.
Ion exchange chromatography
50 mM Tris-HCl pH 7.5, 4 M Urea, 150 mM NaCl
-70°C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
(Catalogue number and MAb: 8G47)
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