Glial Fibrillary Acidic Protein, Antibody

4G25

GFAP15cc, GFAP81cc, GFAP83cc, GFAP94cc, GFAP98cc, GF5
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with native human GFAP (MAbs GFAP81cc, GFAP83cc, GFAP94cc and GFAP98cc, GF5) or with synthetic peptide ₁AGFKETRASERAEMME₁₆ corresponding to 60-75 a.a.r. of GFAP conjugated with a carrier protein (MAb GFAP15cc).

Human GFAP

IgG1 for MAbs GFAP15cc, GFAP81cc, GFAP83cc, GFAP94cc, GFAP98cc
IgG2b for MAb GF5

Recommended pairs for sandwich immunoassay (capture – detection): GFAP83cc – GFAP81cc, GFAP94cc – GFAP98cc, GFAP15cc – GFAP81cc
MAbs GFAP15cc, GFAP81cc, GFAP83cc, GFAP94cc and GFAP98cc are recommended for Western blotting.
MAbs GFAP15cc, GFAP81cc, GFAP83cc and GF5 are recommended for immunohistochemistry.
MAb GF5 is working in ELISA and indirect immunofluorescence staining.

Chromatography on protein A Sepharose (MAbs GFAP15cc, GFAP81cc, GFAP83cc, GFAP94cc, GFAP98cc)
Chromatography on protein G Sepharose (MAb GF5)

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

+4 °C (+2 … +8 °C allowed)

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.