Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with dopamine conjugated with bovine serum albumin.
Affinity of MAb 2B11:
Kd = 10⎺8 for DA-BSA
Kd = 10⎺7 for NA-BSA
Kd = 10⎺4 for unconjugated DA
At 1 µM MAb 2B11 inhibits dopamine binding to D-2 receptor in membrane P-2 fraction from human Corpus striatum.
IgG1 for MAb 2B11
Immunocytochemistry detection of catecholamine-secreted cells.
Chromatography on protein G Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.