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Canine recombinant NT-proBNP (amino terminal fragment of brain natriuretic peptide precursor) Cat.#

8CNT9

Data Sheet

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Properties
Cat.#

8CNT9

Source:

Expressed in E. coli

Application:

Standard for the calibration of canine NT-proBNP immunoassays

Analysis:

Recombinant NT-proBNP corresponds to the fragment 1-85 a.a.r. of canine proBNP and contains additional affinity tag sequence of 16 a.a.r. at the N-terminus.

Totally, recombinant NT-proBNP consists of 101 a.a.r.

Predicted molecular mass 10545 Da is confirmed by MALDI-MS.

Purity > 95 % (Tricine-SDS-PAGE).

Concentration is determined spectrophotometrically.

Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of canine NT-proBNP molecule (Cat.# 4CNT5).

Purification:

Purified using tag affinity chromatography

Presentation:

10 mM potassium phosphate, pH 7.4, 150 mM NaCl.

Storage:

-70 °C (-65 … -80 °C allowed)

Other information:

Avoid repeated freezing and thawing.

It is recommended to aliquot the product after the first thawing. To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine serum albumin).

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Additional product information:

Canine NT-proBNP TechNotes
Veterinary product info

References:

Search references from PubMed

Download datasheet as a PDF file below
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