Canine recombinant NT-proBNP (amino terminal fragment of brain natriuretic peptide precursor)

Cat.# 8CNT9

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Veterinary
  • Cat.#

    8CNT9

  • Source:

    Expressed in E. coli

  • Application:

    Standard for the calibration of canine NT-proBNP immunoassays

  • Analysis:

    Recombinant NT-proBNP corresponds to the fragment 1-85 a.a.r. of canine proBNP and contains additional affinity tag sequence of 16 a.a.r. at the N-terminus.

    Totally, recombinant NT-proBNP consists of 101 a.a.r.

    Predicted molecular mass 10545 Da is confirmed by MALDI-MS.

    Purity > 95 % (Tricine-SDS-PAGE).

    Concentration is determined spectrophotometrically.

    Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of canine NT-proBNP molecule (Cat.# 4CNT5).

  • Purification:

    Purified using tag affinity chromatography

  • Presentation:

    10 mM potassium phosphate, pH 7.4, 150 mM NaCl.

  • Storage:

    -70 °C (-65 … -80 °C allowed)

  • Other information:

    Avoid repeated freezing and thawing.

    It is recommended to aliquot the product after the first thawing. To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine serum albumin).

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

  • References:

    Search references from PubMed

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Canine recombinant NT-proBNP (amino terminal fragment of brain natriuretic peptide precursor) (MAb: )

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