TSH, human, antibody

Cat.# 2TS11 / 2TS11cc

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Hormones
  • Cat.#

    2TS11 / 2TS11cc

  • MAbs in vitro (Cat.# 2TS11cc)

    7G12cc, 11E4cc, 10C7cc

  • MAbs in vivo (Cat.# 2TS11)

    TSB1, TSB4, 1CT1, 7CT8

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with TSH from human pituitary gland.

  • Specificity:

     7G12cc: 10C7cc:: whole molecule of human TSH

    TSB1, TSB4: beta-subunit of human

    TSH11E4cc, 1CT1, 7CT8: beta-subunit of human and canine

    TSH10C7cc, 11E4cc, 7G12cc, TSB1, 1CT1 and 7CT8 do not cross-react with human LH, FSH and HCG.

    TSB4 does not cross-react with human LH and FSH, but it cross-reacts with human HCG.

  • MAb isotypes:

    IgG1 for 7G12cc, 11E4cc, 10C7cc TSB1, TSB4, 1CT1, 7CT8

  • Applications:

     Recommended pairs for sandwich ELISA (capture – detection):

    11E4cc – 7G12cc (human TSH)

    TSB1 – TSB4 (human TSH)

    7CT8 – 1CT1 (human and canine TSH)

    11E4cc – 1CT1 (beta-subunit of human and canine TSH)

    MAb 11E4cc reacts with beta-subunit of human and canine TSH in Western blotting under non-reducing conditions.

    MAbs 1CT1 and 11E4cc react with beta-subunit of canine TSH in Western blotting under non-reducing conditions

  • Purification:

    Protein A chromatography for 7G12cc, 11E4cc, 10C7cc, 1CT1, 7CT8

    Ion exchange chromatography for MAbs TSB1, TSB4

  • Presentation:

     PBS, pH 7.4, 0.09 % sodium azide (NaN3) for 7G12cc, 11E4cc, 10C7cc, 1CT1, 7CT8

    0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN3) for TSB1, TSB4

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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TSH, human, antibody (MAb: )

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TSH, human, antibody (MAb: )

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