TSH, human, antibody

Cat.# 2TS11

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Hormones

Alternatives in vitro!
Please note that MAbs 7G12, 11E4 and 10C7 are now available only as an in vitro produced products under Cat.#2TS11cc.

  • Cat.#

    2TS11

  • MAbs:

    TSB1, TSB4, 1CT1, 7CT8

    (MAbs 11E4, 7G12 and 10C7 are available under Cat.# 2TS11cc)

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with TSH from human pituitary gland.

  • Specificity:

    All MAbs specifically recognize the beta-subunit of human TSH.
    MAbs 1CT1 and 7CT8 specifically recognize the beta-subunit of canine TSH.
    MAbs TSB1, 1CT1 and 7CT8 do not cross-react with human LH, FSH and HCG.
    MAb TSB4 does not cross-react with human LH and FSH, but it cross-reacts with human HCG.

  • MAb isotypes:

    IgG1 for MAbs TSB1, TSB4, 1CT1, 7CT8

  • Applications:

    Recommended pairs for sandwich ELISA (capture - detection):
    Pair TSB1 - TSB4 recognizes human TSH
    Pairs 7CT8 – 1CT1 and 11E4cc – 1CT1 recognize β-subunit of human and canine TSH.
    MAbs 1CT1 and 11E4cc react with β-subunit of canine TSH in Western blotting under non-reducing conditions.
    (Please note that MAb 11E4cc is under Cat.# 2TS11cc)

  • Purification:

    Ion exchange chromatography for MAbs TSB1, TSB4
    Chromatography on protein A Sepharose for MAbs 1CT1, 7CT8

  • Presentation:

    0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN₃) for MAbs TSB1, TSB4
    PBS, pH 7.4, 0.09 % sodium azide (NaN₃) for MAbs 1CT1, 7CT8

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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TSH, human, antibody (MAb: )

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