Rat skeletal muscle.
Purity more than 95 % (SDS-PAGE).
Protein content determined by the Lowry procedure.
Immunological identity confirmed by reaction with monoclonal antibody that is specific for the TnI skeletal muscle isoform.
Lyophilized from 0.01 M HCl.
It is recommended to reconstitute this product with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol) to a concentration close to 1 mg/ml.
Solubility is limited at neutral pH and physiological salt concentration.
Lyophilized -20°C (-15 … -30 °C allowed)
Reconstituted -70°C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.