Human skeletal muscle.
Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.
Negative control for cardiac TnI specific antibodies, immunogen for antibody production, calibrator and standard preparation, troponin biochemical and immunochemical studies.
Purity more than 95 % (SDS-PAGE).
Protein content determined by the Lowry procedure.
Immunological identity confirmed by reaction with monoclonal antibody that is specific for the TnI skeletal muscle isoform.
Lyophilized from 0.01 M HCl.
It is recommended to reconstitute this product with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol) to a concentration close to 1 mg/ml.
Solubility is limited at neutral pH and physiological salt concentration.
Lyophilized -20°C (-15 … -30 °C allowed)
Reconstituted -70°C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
(Catalogue number and MAb: 8T25-)