Human skeletal muscle troponin I (skTnI) Cat.#

8T25

Data Sheet

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Properties
Unit

mg
Cat.#

8T25
Source:

Human skeletal muscle.

Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.
Applications:

Negative control for cardiac TnI specific antibodies, immunogen for antibody production, calibrator and standard preparation, troponin biochemical and immunochemical studies.
Analysis:

Purity more than 95 % (SDS-PAGE).

Protein content determined by the Lowry procedure.

Immunological identity confirmed by reaction with monoclonal antibody that is specific for the TnI skeletal muscle isoform.
Presentation:

Lyophilized from 0.01 M HCl.

It is recommended to reconstitute this product with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol) to a concentration close to 1 mg/ml.

Solubility is limited at neutral pH and physiological salt concentration.
Storage:

Lyophilized -20°C (-15 … -30 °C allowed)
Reconstituted -70°C (-65 … -80 °C allowed)
Other information:

Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
References:

See other cardiac markers and blood coagulation products.
See other Troponin references here and here.
Search Troponin I references from PubMed.
Additional product information:

Cardiac troponin I Booklet
Troponin I product information
Download datasheet as a PDF file below