Expressed in E. coli
Synthetic DNA fragment encoding amino acids 28-110 of human cardiac Troponin I (Accession number UniProtKB P19429 (TNNI3_HUMAN)) a (GGGGS)4 linker and amino acids 2-161 of human cardiac/slow skeletal isoform of Troponin C (Uniprot ID: P63316, TNNC1_HUMAN) was used for recombinant protein expression. May contain additional N-terminal methionine residue (in comparison with native human cardiac troponin I).
Does not contain tags.
Theoretical MW: 29072 Da
Theoretical pI: 4.46
This product is suitable for use as a standard and/or calibrator for cTn assay development
Affinity chromatography, ion exchange chromatography
50 mM Tris-HCl, 0.7 M KCl, 5 mM CaCl2 , 0.1% 2-mercaptoethanol, pH 7.5
-70 °C (-65 ... -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.
Should be diluted in a buffer containing BSA if diluted below 1 mg/ml concentration. Recommended concentration for BSA 7.5%.
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
(Catalogue number and MAb: 8IFC20)