Monoclonal mouse anti-troponin C (TnC) Cat.#

4T27

Data Sheet

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MAbs
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Alternative in vitro!
Please note that MAb 7B9 is also available as in vitro produced product under Cat.#4T27cc. For immunoassays utilizing this MAb, we recommend selecting the in vitro produced product.

Properties
Unit

mg

Cat.#

4T27

MAbs:

7B9, 1A2

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human  cardiac troponin complex  (MAb 7B9) or isolated slow skeletal isoform of TnC (MAb 1A2).

Specificity:

Human TnC. No cross-reactivity with cTnT and cTnI. All MAbs recognize TnC also in binary complex with cTnI.

MAb isotypes:

IgG1 for MAb 7B9
IgG2a for MAb 1A2

Applications:

TnC immunoassay and immunoaffinity purification.

All MAbs work in Western blotting.

Best pair for TnC sandwich immunoassay (capture - detection): 1A2 – 7B9

MAbs can be used in high sensitivity cTnI sandwich immunoassay in pairs with antibodies specific to human cTnI (Cat.# 4T21) and Tn complex (Cat.# 4TC2).

Best pairs for Tn complex sandwich immunoassay (capture - detection): Tcom8 (cat #4TC2) – 7B9

Purification:

Chromatography on protein A Sepharose

Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN)

Storage:

+4 °C (+2 … +8 °C allowed)

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. 

Additional product information:

Troponin booklet
Antibodies Produced in vitro

References:

See other cardiac and blood coagulation products.
See other Troponin References.
Search Troponin C references from PubMed.

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