Transferrin, human, antibody

Cat.# 4T15

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Blood coagulation and anemia
  • Cat.#

    4T15

  • MAbs:

    2A2, 1C10, 8B9, 11D3, 12A6

    Hybridoma clones for MAbs 2A2 and 1C10 have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a Balb/c mice immunized with purified transferrin from pooled human plasma.

    Hybridoma clones for MAbs 8B9, 11D3 and 12A6. have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with placental transferrin receptor or transferrin.

  • Specificity:

    Human transferrin for all MAbs.

    Complex between transferrin and soluble transferrin receptor for MAbs 8B9 and 11D3.

    MAb 2A2 reacts with N-terminal domain of human transferrin. MAbs 2A2 and 1C10 do not cross-react with mouse, rabbit, bovine transferrin and with human lactoferrin.

  • MAb isotypes:

    IgG1 for MAbs 2A2, 1C10, 8B9, 11D3, 12A6

  • Applications:

    Detection of transferrin in serum by sandwich immunoassay. Detection of transferrin - transferrin receptor complex by sandwich ELISA. All MAbs are working in ELISA.

    Recommended pairs for transferrin detection by sandwich immunoassay (capture - detection):

    8B9 – 11D3
    8B9 – 12A6
    1C10 – 2A2
    1C10 – 12A6
    11D3 – 1C10
    11D3 – 8B9

    Recommended MAbs for transferrin-transferrin receptor complex detection by ELISA sandwich method using anti-transferrin receptor MAb 23D10 (cat.No # 4Tr26) as coating:

    23D10 – 8B9, 23D10 – 11D3

    MAbs 2A2 and 1C10 inhibit proliferation of Con-A stimulated peripheral blood lymphocytes.

  • Purification:

    Chromatography on protein A Sepharose for MAbs 8B9, 11D3 and 12A6, and chromatography on protein G Sepharose for MAbs 2A2 and 1C10

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

  • Additional product information:

    Detection of Transferrin - Transferrin Receptor Complex TechNotes

  • References:

    See all Transferrin receptor references.
    Search other references from PubMed.

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Transferrin, human, antibody (MAb: )

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