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Monoclonal mouse anti-transferrin Cat.#

4T15

Data Sheet

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Properties
Unit

mg
Cat.#

4T15
MAbs:

2A2, 1C10, 8B9, 11D3, 12A6

Hybridoma clones for MAbs 2A2 and 1C10 have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a Balb/c mice immunized with purified transferrin from pooled human plasma.

Hybridoma clones for MAbs 8B9, 11D3 and 12A6. have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with placental transferrin receptor or transferrin.
Specificity:

Human transferrin for all MAbs.

Complex between transferrin and soluble transferrin receptor for MAbs 8B9 and 11D3.

MAb 2A2 reacts with N-terminal domain of human transferrin with Kd=2.85 x 10⎺11 M. MAbs 2A2 and 1C10 do not cross-react with mouse, rabbit, bovine transferrin and with human lactoferrin.
MAb isotypes:

IgG1 for all MAbs
Applications:

Detection of transferrin in serum by sandwich immunoassay. Detection of transferrin - transferrin receptor complex by sandwich ELISA. All MAbs are working in ELISA.

Recommended pairs for transferrin detection by sandwich immunoassay (capture - detection):

8B9 – 11D3
8B9 – 12A6
1C10 – 2A2
1C10 – 12A6
11D3 – 1C10
11D3 – 8B9

Recommended MAbs for transferrin-transferrin receptor complex detection by ELISA sandwich method using anti-transferrin receptor MAb 23D10 (cat.No # 4Tr26) as coating:

23D10 – 8B9, 23D10 – 11D3

At 10⎺6 M MAbs 2A2 and 1C10 inhibit proliferation of Con-A stimulated peripheral blood lymphocytes.
Purification:

Chromatography on protein A Sepharose for MAbs 8B9, 11D3 and 12A6, and chromatography on protein G Sepharose for MAbs 2A2 and 1C10
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:

Detection of Transferrin - Transferrin Receptor Complex TechNotes
References:

See all Transferrin receptor references.
Search other references from PubMed.
Download datasheet as a PDF file below