2C8, 1D9, F6C5
Hybridoma clones have been derived from hybridization of X63.Ag8.653 myeloma cells with spleen cells of Balb/c mice immunized with human recombinant tumor necrosis factor of alpha type.
MAb F6C5 is directed to different TNF-α epitope than MAbs 2C8 and 1D9. MAbs 2C8 and 1D9 are effective in inhibition of the biological activity of recombinant and natural TNF-α.
IgG1 for MAbs 2C8, 1D9, F6C5
MAbs 1D9 and 2C8 inhibit biological activity of TNF-α.
Recommended pair for sandwich immunoassay (capture - detection): 2C8 - F6C5.
MAbs 2C8, 1D9 and F6C5 work in immunohistochemistry.
Chromatography on protein A Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.