Hybridoma clones have been derived from hybridization of X63.Ag8.653 myeloma cells with spleen cells of Balb/c mice immunized with human recombinant tumor necrosis factor of alpha type.
MAb F6C5 is directed to different TNF-alfa epitope than MAb 2C8. MAb 2C8 is effective in inhibition of the biological activity of recombinant and natural TNF-alfa.
IgG1 for MAbs 2C8, F6C5
MAbs 2C8 inhibit biological activity of TNF-α.
Recommended pair for sandwich immunoassay (capture - detection): 2C8 - F6C5.
MAbs work in immunohistochemistry.
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
(Catalogue number and MAb: 4T10-)