Thyroxine human antibody

Cat.# 2T6

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Hormones
  • Cat.#

    2T6

  • MAbs:

    1H1, XM212

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with conjugate of purified thyroxine (Sigma T-2501) with BSA.

  • Specificity:

    Thyroxine (T4)

    MAbs react with conjugate T4-BSA and with free thyroxine in ELISA. Cross-reactivity with triiodothyronine (Sigma T-2752) is less than 0.1%.

    The reactivity of the MAb XM212 with L-thyroxine is 100 % and with D-thyroxine 30 %.

  • MAb isotypes:

    IgG1 for MAb 1H1
    IgG2a     for MAb XM212

  • Applications:

    Detection of thyroxine. ELISA.

  • Purification:

    1H1 by chromatography on protein A Sepharose
    XM212 by ion exchange chromatography

  • Presentation:

    1H1 in PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

    XM212 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05 % of sodium azide (NaN₃).

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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Thyroxine human antibody (MAb: )

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