Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with conjugate of purified thyroxine (Sigma T-2501) with BSA.
MAbs react with conjugate T4-BSA and with free thyroxine in ELISA. Cross-reactivity with triiodothyronine (Sigma T-2752) is less than 0.1%.
The reactivity of the MAb XM212 with L-thyroxine is 100 % and with D-thyroxine 30 %.
IgG1 for MAb 1H1
IgG2a for MAb XM212
Detection of thyroxine. ELISA.
1H1 by chromatography on protein A Sepharose
XM212 by ion exchange chromatography
1H1 in PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
XM212 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05 % of sodium azide (NaN₃).
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.