S8E4, S8C12, S10D4, S3E11
No cross-reactivity with egg white avidin. Affinity (Kd) > 1 x 108 for all MAbs. MAbs bind with high efficiency with different epitopes of streptavidin molecule. MAb S8C12 reacts with the determinant nonoverlapping with S8E4-, S10D4- and S3E11-epitopes. All MAbs bind to the streptavidin without interfering with the biotin-binding reaction.
IgG1, kappa for all MAbs
MAbs can be used to localize streptavidin, to amplify signals in immunochemistry, immunohistochemistry, ELISA, flow cytometry and immunoblotting. These mouse anti-streptavidin MAbs applied to the ABC complex, previously bound to the second antibody system allow for further enhancement of signal when anti-mouse antibody conjugate is added. MAbs are effective in immunoadsorbtion procedures of streptavidin - biotinylated protein complexes.
Protein A chromatography
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
(Catalogue number and MAb: 3ST10-)