SmarTaq DNA Polymerase is complex mixture of a thermostable 94 kDa BioTaq DNA Polymerase purified from E. coli PVG-AI recombinant strain expressing Thermus aquaticus polymerase gene and specific monoclonal antibodies.
SmarTaq is inactive under conditions of amplification reaction preparation. It provides improved specificity when compared to standard DNA polymerases. SmarTaq can eliminate amplification artefacts such as primer-dimer formation and mis-priming.
An advantage of SmarTaq is the absence of additional heating step for polymerase activation. Heat activation of enzyme occurs during the first denaturation step. An active complex of SmarTaq dissociates automatically over +70 °C, allowing activation of DNA polymerase.
20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50 % glycerol, 0.5 % Nonidet P-40, 0.5 % Tween-20.
One unit of activity is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 74 °C.
NH4-buffer: 166 mM (NH4)₂SO4, 670 mM Tris-HCl (pH 8.8 at 25 °C), 0.1 % Tween-20.
Highly specific PCR, multiplex PCR (highly recommended), high sensitivity applications.
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.
-20 °C (-15 … -30 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.