Expressed in a mammalian cell line
Spike RBD (receptor-binding domain) is a fragment Arg319-Phe541 of SARS-CoV-2 Spike surface glycoprotein [Severe acute respiratory syndrome coronavirus 2] GenBank: QHD43416.1.
Contains C-terminal His6-tag.
Theoretical MW 25921 Da including His6-tag.
Theoretical pI: 8.91.
Antigen for detection of anti-(SARS-CoV-2 Spike RBD) antibodies
Purity > 95%
Concentration is determined spectrophotometrically at A280 using extinction coefficient 1.3.
Metal affinity chromatography
Lyophilized from PBS, pH 7.4
It is recommended to reconstitute this product with deionized water to its initial concentration.
Lyophilized at -20°C (-15 … -30 °C allowed)
Reconstituted at -70°C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
(Catalogue number and MAb: 8COV1-)