S100 proteins, Human, antibody

Cat.# 4S37

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Neuroscience
  • Cat.#

    4S37

  • MAbs:

    8B10, 6G1, 3B10, 4B3

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human brain S100 proteins.

  • Specificity:

    MAbs 8B10, 6G1 and 4B3 are specific to S100BB and S100A1B.

    MAb 3B10 is specific to S100BB.

  • Applications:

    S100 immunoassay and immunoaffinity purification (except MAb 4B3). 

    All MAbs are working in Western blotting.

    Recommended pairs for sandwich immunoassay (capture-detection): 

    8B10 – 6G1
    3B10 – 6G1

    MAbs 8B10, 3B10 and especially 6G1 are sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5 mM CaCl2 in the assay buffers.

  • MAb isotypes:

    IgG1 for MAbs 8B10, 6G1
    IgG2a for MAbs 3B10, 4B3

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN3)

  • Purification:

    Chromatography on protein A Sepharose

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product

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S100 proteins, Human, antibody (MAb: )

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