8B10, 6G1, 3B10, 4B3
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human brain S100 proteins.
MAbs 8B10, 6G1 and 4B3 are specific to S100BB and S100A1B.
MAb 3B10 is specific to S100BB.
S100 immunoassay and immunoaffinity purification (except MAb 4B3).
All MAbs are working in Western blotting.
Recommended pairs for sandwich immunoassay (capture-detection):
8B10 – 6G1
3B10 – 6G1
MAbs 8B10, 3B10 and especially 6G1 are sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5 mM CaCl2 in the assay buffers.
IgG1 for MAbs 8B10, 6G1
IgG2a for MAbs 3B10, 4B3
PBS, pH 7.4, 0.09 % sodium azide (NaN3)
Chromatography on protein A Sepharose
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product