Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human brain S100 proteins.
MAbs 8B10cc, 6G1cc and 4B3 are specific to S100BB and S100A1B.
MAb 3B10 is specific to S100BB.
IgG1 for MAbs 8B10cc, 6G1cc
IgG2a for MAbs 3B10, 4B3
S100 immunoassay and immunoaffinity purification (except MAb 4B3).
All MAbs are working in Western blotting.
Recommended pairs for sandwich immunoassay (capture-detection):
8B10cc – 6G1cc
3B10 – 6G1cc
MAbs 8B10cc, 3B10 and especially 6G1cc are sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5 mM CaCl2 in the assay buffers.
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Protein A chromatography
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product