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Monoclonal mouse anti-human S100 proteins Cat.#

4S37

Data Sheet

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Properties
Unit

mg

Cat.#

4S37

MAbs:

8B10, 6G1, 3B10, 4B3

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human brain S100 proteins.

Specificity:

MAbs 8B10, 6G1 and 4B3 are specific to S100BB and S100A1B.

MAb 3B10 is specific to S100BB.

MAb isotypes:

IgG1 for MAbs 8B10 and 6G1
IgG2a for MAbs 3B10 and 4B3

Applications:

S100 immunoassay and immunoaffinity purification (except MAb 4B3).

All MAbs are working in Western blotting.

Recommended pairs for sandwich immunoassay (capture - detection):

8B10 - 6G1
3B10 - 6G1

MAbs 8B10, 3B10 and especially 6G1 are sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5 mM CaCl in the assay buffers.

Purification:

Chromatography on protein A Sepharose

Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN)

Storage:

+4 °C (+2 … +8 °C allowed)

Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Download datasheet as a PDF file below