S100 proteins, Human, antibody

Cat.# 4S37

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  • Cat.#

    4S37

  • MAbs in vitro

    8B10cc, 6G1cc

    Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

  • MAbs in vivo

    3B10, 4B3

    Mouse monoclonal antibody produced in ascites. Hybridoma clone derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

  • Immunogen:

    Human brain S100 proteins

  • Specificity:

    8B10cc, 6G1cc and 4B3 are specific to S100BB and S100A1B.
    3B10 is specific to S100BB.

  • MAb isotypes:

    IgG1 for 8B10cc, 6G1cc
    IgG2a for 3B10, 4B3

  • Applications:

    S100 immunoassay and immunoaffinity purification (except 4B3).

    MAbs are working in Western blotting.

    Recommended pairs for sandwich immunoassay:

    8B10cc, 3B10 and especially 6G1cc are sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5 mM CaCl2 in the assay buffers.

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

  • Purification:

    Protein A chromatography

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product

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S100 proteins, Human, antibody () —
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