Expressed in an insect cell line
Soluble extracellular domain of human TPO (amino acid residues 19-846). Molecular weight 91981 Da. Does not contain any tags.
To be used as an antigen in the assays for the detection of human TPO-specific autoantibodies.
Purity > 95 % (SDS-PAGE)
Immunoreactivity of recombinant human TPO is checked in ELISA with blood samples from the patients with anti-TPO autoantibodies.
Affinity and ion exchange chromatography
Lyophilized from 20 mM Tris-HCl, pH 8.0, 250 mM NaCl, 0.1 mM KI, 5% D-mannitol.
It is recommended to reconstitute this product with deionized water to its initial concentration.
Lyophilized -20°C (-15 … -30 °C allowed)
Reconstituted -70°C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
It is recommended to use 20 mM Tris-HCl, pH 8.0, 250 mM NaCl, 0.1 mM KI, to prepare diluted samples.
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
(Catalogue number and MAb: 8RTP0-)