Expressed in an insect cell line
Soluble extracellular domain of human TPO (amino acid residues 19-846). Molecular weight 91981 Da. Does not contain any tags.
To be used as an antigen in the assays for the detection of human TPO-specific autoantibodies.
Purity > 95 % (SDS-PAGE)
Immunoreactivity of recombinant human TPO is checked in ELISA with blood samples from the patients with anti-TPO autoantibodies.
Affinity and ion exchange chromatography
Lyophilized from 20 mM Tris-HCl, pH 8.0, 250 mM NaCl, 0.1 mM KI, 5% D-mannitol.
It is recommended to reconstitute this product with deionized water to its initial concentration.
Lyophilized -20°C (-15 … -30 °C allowed)
Reconstituted -70°C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
It is recommended to use 20 mM Tris-HCl, pH 8.0, 250 mM NaCl, 0.1 mM KI, to prepare diluted samples.
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.