Expressed in a mammalian cell line
Soluble human lipoprotein-associated phospholipase A2 (Lp-PLA2), 420 amino acid residues
Theoretical molecular weight (calculated from the sequence) 49 000 Da
Contains 6×His tag on C-terminus
Theoretical pI 7.1
Calibrator or standard in immunoassays
Purity > 75 % (SDS-PAGE). Apparent molecular weight 45-65 kDa (due to glycosylation).
Affinity chromatography with additional chromatographic steps
Lyophilized from 25 mM Na-phosphate, 0.3 M NaCl, pH 7,5 and 100 mM D-mannitol, 0.6% CHAPS.
Reconstitute with deionized water to initial concentration. Do not reconstitute to higher than initial concentration.
-20 °C (-15 … -30 °C allowed)
Avoid repeated freezing and thawing.
It is recommended to aliquot the product after reconstitution.
To prepare diluted samples of Lp-PLA2 it is recommended to use lyophilization buffer.
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.