Expressed in a mammalian cell line
Synthetic DNA fragment encoding whole human interleukin-6 (Accession number UniProtKB P05231, IL6_HUMAN) with 6His-tag on C-terminus was used for protein expression.
Contains C-terminal His6 tag
Theoretical molecular weight 21 950 Da
Theoretical pI 6.77
Calibrator in immunoassay
Concentration is determined spectrophotometrically at A280.
Metal affinity chromatography
PBS, pH 7.4, 0.1% Tween 80
-70°C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
(Catalogue number and MAb: 8IL6)