Monoclonal mouse anti-rat proinsulin Cat.#
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Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragment of rat proinsulin conjugated with a carrier protein.
IgG1 for MAb CCI-17
MAb recognizes rat proinsulin in ELISA and Sandwich type immunoassay. MAb binds specifically to proinsulin and does not bind to insulin or C-peptide.
For sandwich immunoassay it is recommended to use MAbs in pairs with MAbs specific to N-terminal part of C-peptides I and II (see also the data sheet for Cat.# 2I3).
The recommended pair for sandwich-type rat proinsulin I and II immunoassay is (capture-detection):
Chromatography on protein A Sepharose
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
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This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
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