Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human PAPP-A/proMBP (heterotetrameric PAPP-A, htPAPP-A) complex.
Human PAPP-A antigen purified from placental blood (heterotetrameric complex, consisting of PAPP-A subunits and proMBP subunits).
IgG2b for MAbs 10E1cc, 10E2cc
Recommended pairs for detection of total PAPP-A (htPAPP-A and/or dPAPP-A) (capture –detection):
10E2cc – 10E1cc
MAb 10E1cc recognizes htPAPP-A, consisting of PAPP-A subunits and proMBP subunits, in nonreducing
conditions in Western blotting.
Chromatography on protein A Sepharose.
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
+4 °C (+2 … +8 °C allowed)
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.