Expressed in a mammalian cell line.
Synthetic DNA fragment encoding amino acids 82 – 1627 of human pregnancy-associated protein A (PAPP-A, Accession number UniProtKB #Q13219; PAPP1_HUMAN) with E483A mutation was used for recombinant protein expression; homodimer.
Contains C-terminal His10-tag.
Theoretical MW: 173762.91 Da including C-terminal His10-tag
Theoretical pI: 5.56
Concentration is determined spectrophotometrically at A280. Abs 1 mg/ml = 1.34
Metal affinity chromatography
Lyophilized from 20 mM Tris-HCl, 0.15 M NaCl, 5% sucrose, pH 7.5
It is recommended to reconstitute this product with deionized water to its initial concentration.
Lyophilized at -20 °C (-15 … -30 °C allowed), reconstituted at -70 °C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.
(Catalogue number and MAb: 8PA1)