Expressed in a mammalian cell line
Synthetic DNA fragment encoding amino acids 21-198 of human NGAL (Accession number UniProtKB P80188, NGAL_HUMAN) was used for recombinant protein expression.
Contains C-terminal Gly4SerHis6 tag.
Theoretical molecular weight 21 515 Da.
Theoretical pI 9.0.
Calibrator and standard preparation, NGAL biochemical and immunochemical studies.
Concentration is determined spectrophotometrically at A280.
NiNTA affinity chromatography
Lyophilized from 20 mM Tris-HCl buffer, pH 7.5, containing 0.15 M NaCl and 0.1% CHAPS.
It is recommended to reconstitute this product with deionized water to its initial concentration.
Lyophilized -20°C (-15 … -30 °C allowed)
Reconstituted -70°C (-65 … -80 °C allowed)
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.