Alternatives in vitro!
Please note that MAbs 4F4 and 5D4 are now available only as in vitro produced products under Cat.# 1E4cc.
E411, XTE4, 4H10
(MAbs 4F4 and 5D4 are available under Cat.# 1E4cc)
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with IgE from myelomatous human serum.
MAb E411 class specific for human IgE, Fc-region specific
MAb XTE4 - epsilon chain specific
IgG for MAb 4H10
IgG1 for MAb XTE4
IgG2a for MAb E411
Detection of total IgE level and IgE antibodies in ELISA.
MAb E411 can be used for coating.
MAb XTE4 can be used in Western blotting.
MAbs E411 and 4H10 by chromatography on protein A Sepharose
MAb XTE4 by ion exchange chromatography
MAbs E411 and 4H10 in PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
MAb XTE4 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05 % sodium azide (NaN₃).
+4 °C (+2 … +8 °C allowed)
Allergol. et Immunopathol. 1978, vol 1, pp. 423-426.
Allergy and Clin. Immunol. News, 1992, vol.4, 21-25.
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.