IgE, antibody

Cat.# 1E4

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Immunology and serology

Alternatives in vitro!
Please note that MAbs 4F4 and 5D4 are now available only as in vitro produced products under Cat.# 1E4cc.

  • Cat.#

    1E4

  • MAbs:

    E411, XTE4, 4H10

    (MAbs 4F4 and 5D4 are available under Cat.# 1E4cc)

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with IgE from myelomatous human serum.

  • Specificity:

    MAb E411 class specific for human IgE, Fc-region specific
    MAb XTE4 - epsilon chain specific

  • MAb isotypes:

    IgG for MAb 4H10
    IgG1
    for MAb XTE4
    IgG2a
    for MAb E411

  • Applications:

    Detection of total IgE level and IgE antibodies in ELISA.
    MAb E411 can be used for coating.
    MAb XTE4 can be used in Western blotting.

  • Purification:

    MAbs E411 and 4H10 by chromatography on protein A Sepharose
    MAb XTE4 by ion exchange chromatography

  • Presentation:

    MAbs E411 and 4H10 in PBS, pH 7.4, 0.09 % sodium azide (NaN)
    MAb XTE4 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05 % sodium azide (NaN).

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Other information:

    Allergol. et Immunopathol. 1978, vol 1, pp. 423-426.
    Allergy and Clin. Immunol. News, 1992, vol.4, 21-25.

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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IgE, antibody (MAb: )

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